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Expressions of gene Fmr1 in rat cortex, hippocampus and thalamus areas after the rapid eyes movement sleep deprivation
FAN Gui-min WU Dong-mei WANG Pei-jun XIONG Hui YANG Ying-feng FAN Hong-ru WANG Jin-tao*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (3) : 328-332.
Expressions of gene Fmr1 in rat cortex, hippocampus and thalamus areas after the rapid eyes movement sleep deprivation
Objective To investigate the expression of gene Fmr1 in rats cortex, hippocampus and hypothalamus areas after the rapid eyes movement (REM) sleep deprivation.
Methods Using the modified multiple platform method (MMPM), 126 rats were randomly and averagely divided into three groups, the normal control group (CC), the environmental control group (TC) and the sleep deprivation group (SD). Each group was detected on day 1, day 2, day 3, day 5, day 7, and day 9, and the sample tissues were extracted from 7 rats at each time point. Immunohistochemistry and RT-PCR were operated to analysis the expression of gene Fmr1.Results The expressions of gene Fmr1 were increased gradually in the cortex and thalamus of the SD group after 3 days (P<0.05), and the expressions in the CC and TC groups had no significant difference (P>0.05). The expressions of gene Fmr1 were decreased gradually in hippocampus for SD after 3 days (P<0.05), and that in the CC and TC groups had no significant difference (P> 0.05). Conclusion The expressions of gene Fmr1 were increased gradually in the cortex and thalamus but decreased in the hippocampus in the SD group after 3 days.
Rapid eyes deprivation / Gene fmr1 / Immunohistochemistry / Rat
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1. (LBH - Z09006) postdoctoral fund in heilongjiang province 2. (D2007-15) natural fund in heilongjiang province
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