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Effects of resveratrol on the expression of Toll-like receptor 4, NF-кB and Caspase-3 in the hippocampus of the status convulsion rat
ZHOU Qin* ZHANG Qin LI Guang-qian
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (3) : 321-327.
Effects of resveratrol on the expression of Toll-like receptor 4, NF-кB and Caspase-3 in the hippocampus of the status convulsion rat
Objective To study the expression of toll-like receptor 4 (TLR4), nuclear factor κB (NF-кB) and Caspase-3 and the change of neuron apoptosis of the hippocampus in the status convulsion rat, and to explore the effect of resveratrol on them.Methods A total of 106 male Sprague-Dawley（SD）rats were randomly divided into the control (A), convulsion (B) and resveratrol treatment (C) groups. Group B was further randomly divided into four subset groups (B1-B4) which were executed at 4h, 24h, 48h, 72h after convulsion discontinued. Continuous epilepticus was induced by injecting lithium chloride and pilocarpine, and group C was daily injected with 30mg/kg resveratrol 30minutes after convulsion stopped for 3 days. TLR4 and NF-κB/p65 proteins were detected by immunohistochemistry(IHC); the expressions of TLR4 and Caspase-3 mRNA were detected by RT-PCR. The neuron apoptosis was observed by TUNEL. Results The IHC staining of TLR4 protein in group B was significantly higher than in group A (P<0.05), and that in group C was much lower than in B4 group (P<0.01). The expression of NF-кB/p65 showed that hippocampal neurons had positive expression in cell nuclei in group B compared with group A (P<0.05), and the expression of NF-кB/p65 protein in group C was much lower than that in group B4 (P<0.01). The mRNA expressions of TLR4 and Caspase-3 in the rat hippocampus of group B were significantly elevated than that in group A (P<0.05), and the tendency was increased gradually, reaching the peak at 72 hours after seizure, and that in group C was much lower than that in B4 group (P<0.01). The TUNEL positive cells in hippocampus CAl of B group were more than that of group A after the SC 24hours(P<0.01)，reached the highest level at the 72nd hour, and that in group C the TUNEL positive cells were lower than that in B4 group (P<0.01). Conclusion The expression of TLR4, NF-кB and Caspase-3 increased after SC. Resveratrol can down regulate the expression of TLR4, NF-кB and Caspase-3 in the hippocampus with pilocarpine-induced seizures, reduced the number of neuronal apoptosis.These results suggest that resveratrol may have a protective effect against the hippocampus damage caused by status convulsion.
Convulsion / Apoptosis / Toll like receptor 4 / Caspase-3 / Resveratrol / Immunohistochemistry / Rat
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