Role of Notch signaling in platelet derived growth factor-BB induced proliferation of human pancreatic adenocarcinoma

doi:10.3969/j.issn.0529-1356.2014.02.017

Acta Anatomica Sinica ›› 2014 ›› Issue (2) : 239-241. DOI: 10.3969/j.issn.0529-1356.2014.02.017

MA Yong-chao FAN Wen-juan WU Hua WANG Fu-qing*

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Abstract

Objective To clarify Notch signaling in platelet derived growth factor-BB(PDGF-BB) induced proliferation of human pancreatic adenocarcinoma (HPAC). Methods After being treated by PDGF-BB and (or) γ-secretase inhibitor DAPT, changes of HPAC cell proliferation were detected by MTT assay; Alterations of Notch level in HPAC cells were measured by Western blotting assay; Variations of capability in cardiolipin synthetic lecithin(CSL) binding to the promoter of target gene hes-1 were identified by chromatin immunoprecipitation (CHIP) assay. Results PDGF-BB facilitates the proliferation of HPAC cells, upregulates Notch level and enhances the capability in CSL binding to the promoter of hes-1. DAPT and PDGFR inhibitor treatment suppresses and invalidates PDGF-BB effection. Conclusion Notch-1 signaling plays an essential role in the modulation of PDGF-BB-induced proliferation of HPAC cells.

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MA Yong-chao FAN Wen-juan WU Hua WANG Fu-qing*. Role of Notch signaling in platelet derived growth factor-BB induced proliferation of human pancreatic adenocarcinoma[J]. Acta Anatomica Sinica. 2014(2): 239-241 https://doi.org/10.3969/j.issn.0529-1356.2014.02.017

References

［1］Torrisani J, Bournet B, Cordelier P, et al. New molecular targets in pancreatic cancer［J］. Bull Cancer, 2008,95(5):503-512.
［2］Taeger J, Moser C, Hellerbrand C, et al. Targeting FGFR/PDGFR/VEGFR impairs tumor growth, angiogenesis, and metastasis by effects on tumor cells, endothelial cells, and pericytes in pancreatic cancer ［J］. Mol Cancer Ther, 2011,10(11):2157-2167. 
［3］Rahbari NN, Schmidt T, Falk CS, et al. Expression and prognostic value of circulating angiogenic cytokines in pancreatic cancer ［J］. BMC Cancer, 2011,11:286.
［4］Yuzawa S, Kano MR, Einama T, et al. PDGFRβ expression in tumor stroma of pancreatic adenocarcinoma as a reliable prognostic marker ［J］. Med Oncol, 2012, 29(4):2824-2830.
［5］Ilagan MX, Kopan R. SnapShot: notch signaling pathway ［J］. Cell, 2007, 128(6):1246. 
［6］Golde TE, Koo EH, Felsenstein KM, et al. γ-Secretase inhibitors and modulators ［J］. Biochim Biophys Acta, 2013,1828(12):2898-2907.
［7］Thomas G. Furin at the cutting edge: from protein traffic to embryogenesis and disease ［J］. Nat Rev Mol Cell Biol,2002,3(10):753-766.
［8］Capaccione KM, Pine SR. The Notch signaling pathway as a mediator of tumor survival ［J］. Carcinogenesis, 2013,34(7):1420-1430. 
［9］Ma YC, Shi C, Zhang YN, et al.The tyrosine kinase c-Src directly mediates growth factor-induced Notch-1 and Furin interaction and Notch-1 activation in pancreatic cancer cells ［J］. PLoS One， 2012,7(3):e33414.