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Expression of galectin-3 in annexin A7 knockdown HeLa cells
LI Xin* WANG Xiao-jie XU Qian CHEN Long LIANG Xiu-jun
Acta Anatomica Sinica ›› 2014 ›› Issue (2) : 234-238.
Expression of galectin-3 in annexin A7 knockdown HeLa cells
Objective To detect the expression of galectin-3（gal-3） in annexin A7 knockdown HeLa cells. Methods The HeLa cells were grouped into an experiment group, a negative group and a blank group. RNA interference technology was used to transfect the siRNA targeted to ANXA7, scrambled siRNA into HeLa cells with lipofectine 2000 without any treatment to the blank group. After transfection for about 48 hours, Western blotting method and PCR method were used to assure the suppression of ANXA7 on both protein level and mRNA level. The expression of galectin-3 was detected with Western blotting and real time qualitative PCR. Results The expression of ANXA7 was significantly suppressed in the cells transfected with ANXA7 siRNA. When ANXA7 expression was suppressed, the expression of galectin-3 decreased significantly compared with the negtive control group and blank control group. The results have significant meaning（P<0.05）. Conclusion The expression of galectin-3 is down-regulated in the ANXA7 suppressed HeLa cells, which may be one of the mechanisms for the abnormal behaviour of the ANXA7 knockdown carcinoma cells.
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