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Effect of XAV939 on proliferation inhibition of human neuroblastoma cell
TIAN Xiao-hong HOU Wei-jian BAI Shu-ling* FAN Jun TONG Hao XU He
Acta Anatomica Sinica ›› 2014 ›› Issue (2) : 190-195.
Effect of XAV939 on proliferation inhibition of human neuroblastoma cell
Objective To study the effect of antiproliferative and apoptosis induction of small molecule inhibitor XAV939 on neuroblastoma (NB) cell line SH-SY5Y cells, and its possible mechanism. Methods The MTT assay was used to detect the inhibition effect of XAV939 on the viability of SH-SY5Y cells, and to determine the optimal concentration. Annexin V-FITC assay was used to detect the percentage of early apoptotic cells after XAV939 treatment. Hoechst33342 staining was used for observing the apoptotic nuclear morphology. Colony forming assay was applied for detecting the proliferation effect of XAV939 on SH-SY5Y cells. The expression of key proteins of Wnt/β-catenin pathway and anti-apoptotic protein Bcl-2 were detected by Western blotting. Results The MTT assay showed that the proliferation inhibition rate of SH-SY5Y cells increased significantly by 1μmol/L XAV939 for 24hours. The percentages of apoptotic cells were significantly higher than those in control groups after treated with XAV939 for 48hours and 72hours, and the cells showed varying degrees of apoptotic morphology. In addition, XAV939 significantly reduced the colony formation rate of SH-SY5Y cells in vitro. The results of Western blotting showed that XAV939 reduced the expression of key proteins of Wnt/β-catenin signaling pathway as well as anti-apoptotic protein Bcl-2. Conclusion XAV939 may inhibit the proliferation of SH-SY5Y cells in part by inhibiting the Wnt/β-catenin signaling pathway.
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