Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Morphological changes and NOS expression of neurons in CA3 hippocampus of rats
LIU Ji*WANG Xiao-yu SUN Yang GUO Xiu-ying PANG Yin
Acta Anatomica Sinica ›› 2014 ›› Issue (2) : 181-184.
Morphological changes and NOS expression of neurons in CA3 hippocampus of rats
Objective To observe morphological changes and the nitric oxide synthase (NOS) expression of neurons in CA3 hippocampus of rats and investigate morphological changes of hippocampal neurons and the role of nitric oxide (NO) in the pathogenesis of hepatic encephalopathy. Methods Fifty male Wistar rats were divided into the control group and experimental group. Before the experiment all the rats were tested by Morris water maze test. After 9 weeks, a CCL4 model of hepatic encephalopathy was established. The hippocampus of each group was taken out for Nissl staining and dyeing of NADPH-d. Results 1.Nissl’s staining: In the experimental group, the number of neurons in hippocampus were less than the control group and dyed lighter, Nissl bodies were also reduced. 2. NADPH-d staining results: In the experimental group, the axon was dyed more deep，widespread dendrites connections were observed. In the control group there were a few positive axons and less connections between dendrites. The color in the experimental group was blue or deep blue and showed positive or strong positive with NADPH-d staining, while in the control group showed weak positive. Conclusion Hepatic encephalopathy can lead to hippocampus injury. NO may mediate the process and the blood ammonia level is one of the pathogenesis of HE.
［1］ Zhu ShQ.Discussion on the precipitating factors of hepatic encephalopathy ［J］. Chinese Journal of Practical Neruous Diseases, 2011, 14( 22):60-61.(in Chinese)
朱顺强.肝性脑病的诱发因素探讨［J］.中国实用神经疾病杂志，2011，14( 22):60-61.
［2］ Luo HM. The structure, function, the hippocampus from morphological plasticity aging change［J］. Chinese Journal of Neuroanatomy, 1996, 12(2): 177-184. (in Chinese)
罗焕敏. 海马结构从形态、功能、到可塑性衰老性变化［J］. 神经解剖学杂志,1996，12(2): 177-184.
［3］ Wang LP, Chen X, Gao J. Research progress of ammonia poisoning involved in oxidative stress and hepatic encephalopathy ［J］. International Journal of Digestive Disease, 2011,31(2):95-96. (in Chinese)
王立鹏，陈晓，高峻.肝性脑病中氧化应激参与氨中毒的研究进展［J］.国际消化病杂志，2011,31(2):95-96.
［4］Kim MA, Jung EJ, Lee HS, et al.P-eadherin expression ingastric carcinoma；its regulation mechanism and prognostic significance［J］.Hum Pathol，2010,41（6）：877-885.［5］Bergen WG, Wu G.Intestinal nitrogen recycling and utilization in health anddisease［J］.J Nutr，2009,139(5)：821-825.
［6] H?ussinger D, G?rg B. Interaction of oxidative stress as-trocyte swelling and cerebral ammonia toxicity［J］.Curr Opin Clin Nutr Metab Care, 2010,13(1)：87-92.
［7］Chastre A, Jiang W, Desjardins P, et al.Ammonia and proinflammatory cytokines modify expressionofgenes codingforastroeytic proteins implicatedin brain edema in acute liverfailure［J］.Metab Brain Dis,2010,25(1)：17-21.
［8］Lockwood AH. Blood ammonia levels and hepatic encephalopathy［J］. Metab Brain Dis,2004, 19(3-4):345-349
［9］Zhang QJ, Liu YY, Liu H, et al. Ammonia in the pathogenesis of hepatic encephalopathy in the role of ［J］. Chinese Journal of Rehabilitation Theory and Practice, 2010,16(2):136-139. (in Chinese)
张清俊,刘雁勇,刘赫,等.氨在肝性脑病发病机制中的作用［J］.中国康复理论与实践,2010,16(2):136-139.
［10］Degroot A, Parent MB. Increasing acetylcholine levels in the hippocampusor entorhinal cortex reverses the impairing effects of septal GABA receptorac tivation on spontaneousal ternation［J］.Learn Mem,2000,7(5):293-302.
［11］Liu J, Zhu JZh, Sui YL, et al. The morphological changes and NOS expression of neurons in dentate gyms hippocampus of rats ［J］. Chinese Journal of Neuroanatomy, 2012, 28(3):294-300. (in Chinese)
刘冀,朱建忠,隋月林,等. 肝性脑病大鼠海马齿状回神经元形态及一氧化氮合酶表达的变化［J］.神经解剖学杂志，2012，28(3):294-300.
［12］Shebani KO, Stucchi AF, McClung JP, et al. Role of stasis and oxidative stress in ileal pouch inflammation［J］.J Surg Res，2000，90（1）: 67-75.
/
| 〈 |
|
〉 |
