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Expression and significance of Nestin and SOX2 in the SAMP8 mice subfornical organ
YE Jian-ya LI Cong-hui TANG Wei-dong MA Chang-sheng HAO Qing-mao QIN Yong*
Acta Anatomica Sinica ›› 2014 ›› Issue (2) : 176-180.
Expression and significance of Nestin and SOX2 in the SAMP8 mice subfornical organ
Objective To observe the expression of nestin and sex-determining genes in high-mobility group proteins in the senescence accelerated mouse prone 8 subfornical organ. Methods Adult SAMP8 mice (8 months old) were used. The expressions of Nestin, SOX2 in subfornical organ were observed by using immunohistochemical staining and immunofluorescence staining methods. Normal senescence mouse resistance 1 was displayed as control. Results Nestin immunohistochemical staining of the control group: positive cells were protuberantly distributed and expressed filiform texture with radiate. Nestin immunohistochemical staining of the experimental group: positive cells body stained stronger, filiform texture was thick, densely distributed in surrounded by vessels. The percentage of positive cells（49.17±7.60）% increased significantly than control group（16.33±4.41）% and had statistical significance (P<0.01). SOX2 immunohistochemical staining of control group: positive cells stained irregularity and dispersedly distributed. SOX2 immunohistochemical staining of the experimental group: most of positive cells stained deeper, densely distributed in surrounded by vessels. The percentage of positive cells（62.17±20.27）% increased significantly than control group（36.00±16.20）% and had statistical significance (P<0.05). Fluorescence double staining in the control group: SOX2 stained irregularity and Nestin expressed during it. A small amount of SOX2 and Nestin double-positive cells were in the subependymal region. In the experimental group: positive cells expressed intensity and showed more double-positive cells. Conclusion Expression of nestin and SOX2 enhances significantly in the senescence accelerated mouse prone 8 subfornical organ, which indicates AD may result in increased number of neural stem/progenitor cell proliferation or differentiation in subfornical organ at same stage，and then the neurogenesis of subfornical organ may be affected in Alzheimer disease.
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