Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Effect of eucalyptus oil on rat embryo implantation and growth interfered with retinoic acid
SU Li-li HE Ying-hong CHEN Mo YU Yong-li* HUANG Yi-ling ZHU Cheng-xue LIU Mao-sheng
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (1) : 114-119.
Effect of eucalyptus oil on rat embryo implantation and growth interfered with retinoic acid
Objective To study the effect of eucalyptus oil on retinoic acid(RA) interference in rat embryo implantation and growth. Methods Forty-two pregnant SD rats were randomly divided into 6 groups,7 rats each: 3 experiment groups ( high, medium, low doses of Eucalyptus+RA), a solvent group (peanut oil +RA), a retinoic acid group (RA) and a normal group with normal diet. Three experiment groups and Solvent group were lavaged from gestation 7th to 14th day with eucalyptus oil in 300 mg/kg, 200 mg/kg, and 100 mg/kg and peanut oil 2ml per animal per day, respectively. Each of them was garaged with retinoic acid (40mg/kg) at gestation 10th day. RA group was garaged with 40 mg/kg at gestation 10th day. All pregnant rats were sacrificed at 21st day. The weights of the body, uterus and placenta of the pregnant rats were measured. The number of absorbed, live and dead fetus were counted respectively. The length of fetus body and tail as well as body weight were measured. Results The uterus and placenta weight, change in body weight of pregnant rats in normal group, Eucalyptus oil groups and Solvent group showed no significant difference compared with RA group. The solvent group rats’ placental weight was lower than that in eucalyptus oil groups’ and RA group’s (P>0.05), but it was significantly lower than that in normal group (P<0.05). The rate of live fetus in normal group was more than that in other groups with significant difference (P<0.05). The teratogenic rate and the absorption rate of normal group were lower than that of the other groups’ (P<0.05). The mortality of normal group was zero. In gavaged retinoic acid groups, the rates of live fetus in high, medium of eucalyptus oil group were higher than that in RA group (P>0.05). It’s teratogenic rate was lower than that of RA group(P>0.05). The absorption rate in each eucalyptus oil group and solvent group compared with RA group had no difference. The fetus body weight, body length, and tail length of Normal group were significantly higher than that in the other groups（P<0.05). In gavaged retinoic acid groups, the fetus body weight and body length of each eucalyptus oil group were significantly higher than that in solvent group and RA group（P<0.05). The fetus body weight, body length, tail length of solvent group had no significant difference compared with the RA group (P>0.05). The fetus tail length in eucalyptus oil groups was higher than that in RA group, but no significant differences were found(P>0.05). Conclusion Retinoic acid interferes the rat embryo implantation and development; Eucalyptus oil has certain influence on the RA interference of rat embryo implantation, and it also has certain inhibition on fetal growth retardation.
Eucalyptus oil / Retimoic acid / Teratogenicity / Embryonic development / Reproductive toxicity testing / Rat
［1］Tian YH, Zhang XM, Huang TS,et al. Research advances on the essential oils from leaves of eucalyptus［J］. Food and Fermentation Industries, 2007，33（10）：139-142.(in Chinese)
田玉红，张祥民,黄泰松, 等.桉叶油的研究进展［J］.食品与发酵工业,2007，33（10）：139-142.
［2］Soares MC, Damiani CE, Moreira CM, et al. Eucalyptol, an essential oil, reduces contractile activity in rat cardiac muscle［J］. Braz J Med Biol Res, 2005,38(3):453-461.
［3］Coelho-de-Souza LN, Leal-Cardoso JH, de Abreu Matos FJ, et al. Relaxant effects of the essential oil of Eucalyptus tereticornis and its main constituent 1,8-cineole on guinea-pig tracheal smooth muscle［J］. Planta Med, 2005,71(12):1173-1175.
［4］Nascimento NR, Refosco RM, Vasconcelos EC, et al. 1,8-Cineole induces relaxation in rat and guineapig airway smooth muscle［J］. Pharm Pharmacol, 2009,61(3):361-366.
［5］Lima-Accioly PM, Lavor-Porto PR, Cavalcante FS, et al. Essential oil of croton nepetaefolius and its main constituent, 1,8-cineole, block excitability of rat sciatic nerve in vitro［J］. Clin Exp Pharmacol Physiol, 2006,33(12):1158-1163.
［6］Ferreira-da-Silva FW, Barbosa R, Moreira-Júnior L, et al. Effects of 1,8-cineole on electrophysiological parameters of neurons of the rat superior cervical ganglion［J］. Clin Exp Pharmacol Physiol, 2009,36(11):1068-1073.
［7］Gomes PB, Feitosa ML, Silva MI, et al. Anxiolytic-like effect of the monoterpene 1,4-cineole in mice［J］. Pharmacol Biochem Behav, 2010, 96(3):287-293.
［8］Yu YL，Wang ChT，Feng GW，et al. Study the protection effects of eucalyptus on the genetic toxicity in mice treated by cyclophosphamide［J］.Chinese Journa of Pharmacology and Toxicology, 2010, 24（6）：525-529. (in Chinese)
余永莉，王传铜，冯国纹,等.桉叶油毒性及其对环磷酰胺致小鼠骨髓微核及精子畸形的保护作用［J］.中国药理与毒理学, 2010, 24（6）：525-529.
［9］Yu YL,Feng GW, Tang YCh, et al. Study the protection effects of eucalyptus on embryonic developmental toxicity in SD rats treated by cyclophosphamide［J］. Chinese Journal of Modern Applied Pharmacy, 2011,19（9）：189-192. (in Chinese)
余永莉，冯国纹，唐于川，等.桉叶油对环磷酰胺致大鼠胚胎毒性的保护作用［J］. 中国现代应用药学, 201123（3）：189-192.
［10］You ShQ, Han Y. Retinoic acid (RA) on mouse embryo toxicity and teratogenicity［J］. Journal of Toxicology, 1994,8(3): 41-42. (in Chinese)
尤胜权,韩锐. 维甲酸(RA)对小鼠的胚胎毒性及致畸胎性［J］.卫生毒理学杂志,1994,8(3): 41-42.
［11］Li Y, Zhang TB. Developmental Toxicology Research Methods and Experimental Techniques［M］. Beijing: Peking University Medical Press, 2000：145.(in Chinese)
李勇，张天宝.发育毒理学研究方法和实验技术［M］.北京:北京医科大学出版社， 2000：145.
［12］Xiong Y, Zeng YSh,Tang JY, et al. The effect of ganoderma spores on the expression of Cdk4 in embryonic neural tube defects induced by retinoic cide. ［J］. Actia Anatomica Sinica, 2007,38（3）：369-372. (in Chinese)
熊轶，曾园山，唐久余，等. 灵芝孢子在降低维甲酸诱导胚胎小鼠发生神经管畸形过程中对Cdk4表达的影响［J］.解剖学报，2007,38（3）：369-372.
［13］Ma YZh. Study of teratogenicity of retinoic acid on neural tube defects of mouse embryo and antagonistic effect of oyster［J］. Reproduction and Contraception, 2011，31(4)：225-229. (in Chinese)
马永臻.牡蛎提取物拮抗维甲酸致小神经管畸形的实验研究［J］.生殖与避孕,2011，31(4)：225-229.
［14］Yang M, Ji H, Dai ShJ, et al Effect of sarsasapogenin on prevention and treatment of retinoic acid induced osteoporosis in mice［J］. Cinese Journal of Natural Medicine, 2006,4(3):219-223. (in Chinese)
杨茗，季晖，戴胜军，等.知母皂苷元对维A酸致小鼠骨质疏松的防治作用［J］. 中国天然药物，2006,4(3):219-223.
［15］Du P, Xiao RM, Chen Y. Effects of epimedium sagittatum flavonoids and eucommia ulmoides flavonoids on osteoporosis mice induced by retinoic acid［J］. Journal of Hubei University（Natural Science Edition）, 2005,(4): 392-394. (in Chinese)
杜鹏,肖润梅,陈勇.淫羊藿总黄酮、杜仲总黄酮对维甲酸所致小鼠骨质疏松的实验研究［J］.湖北大学学报：自然科学报, 2005,(4): 392-394.〖ZK)〗
［16］Yu YL, Tang YCh, Feng GW, et al. Study the effect of eucalyptus on embryonic development in rat［J］. Actia Anatomica Sinica, 2010, 41（6）：916-918. (in Chinese)
余永莉，唐于川，冯国纹, 等.桉叶油对大鼠胚胎发育的影响［J］.解剖学报, 2010, 41（6）：916-918.
/
| 〈 |
|
〉 |
