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Effect of testosterone on the in vitro mouse follicle development
MU Xiao-li LIU Tao LIU Hong-bin GAO Yuan GAO Xuan GENG Ling YOU Li XU Cheng-yan*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (1) : 104-108.
Effect of testosterone on the in vitro mouse follicle development
Objective To investigate the effect of testosterone on the in vitro mouse follicle development. Methods We chose follicles of 12-day-old ICR female mice culturedin vitro as our research models. Follicles were divided into four groups randomly with thirty follicles in each group and were treated with different concentration testosterone: 0(control group), 10-4 mol/L,10-5 mol/L,10-6 mol/L. Follicles were divided into three groups randomly with thirty follicles in each group and were treated with different reagents: no treatment (control group), 10-5 mol/L testosterone(group T), 10-5 mol/L androgen antagonist flutamide pretreatment plus 10-5 mol/L testosterone(group F+T). Follicles were cultured on microdrop. We observed follicle outlines, granulosa cells proliferation and oocytes morphology under the inverted microscope every day, recorded follicle degradation. Results The development of follicles was inhibited in the group treated with 10-4 mol/L testosterone. While the granulosa cells proliferation and the development of follicles were promoted in the group treated with 10-5 mol/L testosterone. Meanwhile, testosterone stimulated the follicles development in the early stage of the follicular development, but it inhibited the follicles development in the late stage. Conclusion 10-5 mol/L testosterone can promote the development of primary follicles and secondary follicles, but it can inhibit the antral follicles development.
Testosterone / Follicle development / In vitro culture / Mouse
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