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Expressions and clinical significance of ROBO4 in both serum and tumor tissues in patients with epithelial ovarian cancer
MA Rui-qiong YE Xue CHENG Hong-yan CHEN Jun CUI Heng WEI Li-hui CHANG Xiao-hong*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (1) : 64-69.
Expressions and clinical significance of ROBO4 in both serum and tumor tissues in patients with epithelial ovarian cancer
Objective To explore the levels of ROBO4 and its roles in patients with ovarian tumor, and to investigate the correlation between the expressions of ROBO4 in ovarian tumor tissues and the related clinicopathological characteristics. Methods We examined expression of ROBO4 in 40 cases of the normal ovarian tissue, 54 cases of the ovarian cyst tissue, and 110 cases of ovarian cancer by using the immunohistochemistry and RT-PCR, and compared with clinicopathological features of ovarian cancer. Serums were checked by ELISA in patients with ovarian cancer and ovarian benign tumors and health women.
Results The intensities of ROBO4 protein immunohistochemical positive staining and its mRNA expression were significantly lower in ovarian cancer than those in both normal ovarian tissues and benign tumor tissues (P<0.05). The serum ROBO4 in patients with malignant ovarian tumor was significantly lower than those in healthy women and benign tumors (P<0.05). Loss or reduced expression of ROBO4 was related with clinical stages, lymph node metastasis or omental metastasis or ascites of ovarian cancer (P<0.05), but not related with age, pathologic types, and histological grade. The correlation analysis showed that there was no correlation between serum ROBO4 and CA125. Conclusion Reduced expression of ROBO4 plays an important role in tumorigenesis of ovarian cancer, which is related to invasion and metastasis of ovarian cancer.
Ovarian carcinomas / ROBO4 / Immunohistochemistry / RT-PCR / Human
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