Regulation of resveratrol to key enzyme in homocysteine metabolism in insulin resistance model

FAN Xiao-ming ZHANG Tie-jun KANG Li LIU Shi-chang GUO Jia-zhi LU Di GUI Li* LI Shu-de*

doi:10.3969/j.issn.0529-1356.2014.01.011

Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (1) : 58-63. DOI: 10.3969/j.issn.0529-1356.2014.01.011

Regulation of resveratrol to key enzyme in homocysteine metabolism in insulin resistance model

FAN Xiao-ming¹ ZHANG Tie-jun¹KANG Li¹ LIU Shi-chang¹GUO Jia-zhi¹ LU Di1 GUI Li ^2* LI Shu-de^3*

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Abstract

Objective To investigate the regulation mechanism of resveratrol(RES) on key enzymes of homocysteine metabolism for SD rats with insulin resistance.
Methods Six weeks healthy mice were made as the insulin resistance model with diet by high fat and sugar and gavage by high fat for 3 months. The rats were randomly divided into the insulin resistance group (n=10)and the resveratrol intervention group (n=10). High fat and sugar were fed in the insulin resistance group for eight weeks. The resveratrol intervention group was fed by high fat and sugar and gavage by resveratrol［30mg/（kg·d)］ for eight weeks. The concentrations of homocysteine, glucose, insulin, total cholesterol and triglyceride were measured in the fasting state. The insulin resistance index (IRI) was calculated. The expressions of methylenetetrahydrofolate reductase (MTHFR), cystathionine β synthase (CBS) and methionine synthetase (MTR) were detected by immunohistochemical, RT-PCR and Western blotting in the rat liver tissue. Results The concentrations of the blood glucose, blood insulin and triglyceride in the resveratrol intervention group were lower than that in the insulin resistance group (P<0.05). The ISI was lower in the resveratrol intervention group. The concentration of homocysteine was decreased (P<0.05). However, the weight and the concentration of total cholesterol were no statistical significance (P>0.05). The protein expressions of MTHFR and CBS in the resveratrol intervention group were higher compared with the insulin resistance group by Western blotting and immunohistochemical staining(P<0.05).The mRNA expressions of MTHER and CBS in the resveratrol intervention group were higher compared with the insulin resistance group by RT-PCR. However, the expression of MTR protein and mRNA were not different between the resveratrol intervention group and the insulin resistance group (P>0.05). Conclusion Resveratrol can improve the expression of the MTHFR and CBS which participate the metabolism of homocysteine and facilitate the transformation of homocysteine, thus it may play a key role in alleviating insulin resistance.

Key words

Insulin resistance / Resveratrol / Homocysteine / Immunohistochemistry / Western blotting / RT-PCR / Rat

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FAN Xiao-ming ZHANG Tie-jun KANG Li LIU Shi-chang GUO Jia-zhi LU Di GUI Li* LI Shu-de*. Regulation of resveratrol to key enzyme in homocysteine metabolism in insulin resistance model[J]. Acta Anatomica Sinica. 2014, 45(1): 58-63 https://doi.org/10.3969/j.issn.0529-1356.2014.01.011

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