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Effects of sericin on Akt signal transduction pathway of hippocampus of the rat with diabetes mellitus
SONG Cheng-jun ZHANG Yan HE Ya-qiang CHEN Zhi-hong*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (1) : 31-36.
Effects of sericin on Akt signal transduction pathway of hippocampus of the rat with diabetes mellitus
Objective To investigate the effects of sericin (obtained by soaking, decocting and concentrating from silkworm cocoon) on Akt signal transduction pathway of hippocampus of the rat with diabetes mellitus. Methods Thirty-six male SD rats were randomly divided into 3 groups, 12 rats per group: normal control group, diabetes mellitus model group and sericin treatment group. The type 2 diabetes mellitus rat model was established by continuous peritoneal injection of streptozotocin with a small dose (25mg/kg/d, 3d). The rats in sericin treatment group were lavaged with sericin for 35d (2.4g/kg·d). TUNEL was used to detect neurons apoptosis in CA1 region of hippocampus; Western blotting and RT-PCR to detect the expression of Akt signal transduction pathway related factors: Akt, NF-κB and Bad. Results Compared with normal control rats, the apoptotic index of hippocampal neurons of diabetes mellitus rats increased obviously (P＜0.01); Akt and NF-κB expression in hippocampus decreased obviously, Bad expression increased obviously (P＜0.01). Compared with diabetes mellitus rats, the apoptotic index of hippocampal neurons of rats in sericin treatment group decreased significantly (P＜0.05); Akt and NF-κB expression in hippocampus increased significantly, Bad expression decreased significantly (P＜0.01, P＜0.05). Conclusion Sericin can reduce hippocampal neurons apoptosis by regulating abnormal changes of Akt signal transduction pathway, thus sericin has fairly protective effects on hippocampus injury during diabetes mellitus.
Sericin / Type 2 diabetes mellitus / Hippocampus / Apoptosis / Akt signal transduction pathway；TUNEL / Rat
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