Welcome to visit Acta Anatomica Sinica! Today is
Chinese
A semi-quantitative RT-PCR analysis for NeuroD mRNA expression in brain during rat development
GUO Wei LIU Xiao-yan KE Li-ning LI Yu-yu LIN Ling XU Jian-wen*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (1) : 26-30.
A semi-quantitative RT-PCR analysis for NeuroD mRNA expression in brain during rat development
Objective To observe the NeuroD mRNA expression during rat neural system development and to investigate its possible roles in neural development and differentiation. Methods Total mRNA was abstracted from different parts of brains in different development stages with Trizol kit in rats. The mRNA of NeuroD and β-actin was reversely transcripted and amplificated by semi-quantitative reverse transcription-polymerase chain reaction method (RT-PCR). The quantity of NeuroD and β-actin mRNA expression was detected by gel imaging system. Results NeuroD mRNA existed in E7.5. The mRNA expression of NeuroD and β-actin peaked in E10.5 and E21.5, which average gray values were 20437.88±598.28 and 14482.23±1134.49, respectively. The mRNA expression of NeuroD and β-actin in E10.5 and E21.5 was significantly higher than other time points (P<0.01). The ratios of NeuroD to β-actin in E12.5,E18.5 and E21.5 were 1.59±0.09、1.61±0.07 and 1.70±0.11 respectively, which were higher than other time points(P<0.01). Conclusion The expression of NeuroD mRNA showed an apparent time-specific pattern during the development of the rat brain. The expression level of neuroD increased obviously in two phases. One was in E10.5, when the original rat brain came into being; the other was the anaphase in neural development, when brain form made the perfection, brain tissue stepped into its maturation. Thus, our results suggested the functions of NeuroD: 1. NeuroD influenced the generation and differentiation of nerve cells; 2. NeuroD played its role in the maturation of nervous system.
Neurogenic differentiation / Basic helix-loop-helix / Neuronal development / Neuronal differentiation / RT-PCR / Rat
［1］Lee JE, Hollenberg SM, Snider L, et al. Conversion of xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein ［J］. Science, 1995, 268(5212): 836-844.
［2］Guo QS, Zhu MY, Wang L, et al. Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells ［J］. Exp Diabetes Res, 2012, 2012:1-10.
［3］Karl MO, Thomas JL, Ochocinska MJ, et al. Using the Tg(nrd:egfp)/albino zebrafish line to characterize in vivo expression of neurod ［J］. PLoS One, 2012, 7(1): e29128.
［4］Wang L, Bluske KK, Dickel LK, et al. Basal progenitor cells in the embryonic mouse thalamus-their molecular characterization and the role of neurogenins and Pax6 ［J］. Neural Dev, 2011, 6:35.
［5］Stevens CB, Cameron DA, Stenkamp DL. Plasticity of photoreceptor-generating retinal progenitors revealed by prolonged retinoic acid exposure ［J］. BMC Dev Biol, 2011, 11:51.
［6］Stephenson DT, O'neill SM, Narayan S, et al. Histopathologic characterization of the BTBR mouse model of autistic-like behavior reveals selective changes in neurodevelopmental proteins and adult hippocampal neurogenesis ［J］. Mol Autism, 2011, 2(1): 7.
［7］Nibu K-I, Li G, Zhang X, et al. Olfactory neuron-specific expression of NeuroD in mouse and human nasal mucosa ［J］. Cell Tissue Res, 1999, 298(3): 405-414.
［8］Suzuki Y, Takeda M, Obara N. Expression of NeuroD in the mouse taste buds ［J］. Cell Tissue Res, 2002, 307(3): 423-428.
［9］Liu XY, Xu JW, Wang W. Expression of neurogenic differentiation factor increased after transient hypoxia of hippocampal neurons in vitro ［J］. Acta Anatomica Sinica, 2011, 42(1):27-31.(in Chinese)
刘晓艳, 徐剑文, 王玮. 短暂性缺氧后海马神经元神经源性分化因子表达增高 ［J］. 解剖学报, 2011, 42(1): 27-31.
［10］Li YY, Xu JW, Guo W, et al. Expression of neurogenic differentiation factor in the brain of transient hypoxia postnatal rats［J］. Acta Anatomica Sinica, 2011, 42(5):610-613. (in Chinese)
李玉宇, 徐剑文, 郭玮, 等. 神经源性分化因子在新生大鼠短暂脑缺氧中的表达 ［J］. 解剖学报, 2011, 42(5): 610-613.
［11］Li YY, Xu JW, Guo W, et al. Effects of transient hypoxia on apoptosis and neurogenesis in the newborn rat brain［J］. Acta Anatomica Sinica, 2011, 42(6): 741-745. (in Chinese)
李玉宇, 徐剑文, 郭玮, 等. 短暂缺氧对新生鼠脑细胞凋亡和神经再生的影响 ［J］. 解剖学报, 2011, 42(6): 741-745.
［12］Henrique D, Chung P-C, Lin W-S, et al. Zebrafish Her8a Is Activated by Su(H)-Dependent Notch Signaling and Is Essential for the Inhibition of Neurogenesis ［J］. PLoS One, 2011, 6(4): e19394.
［13］Schlosser G. Hypobranchial placodes in Xenopus laevis give rise to hypobranchial ganglia, a novel type of cranial ganglia ［J］. Cell Tissue Res, 2003, 312(1):21-29.
［14］Murai K, Philpott A, Jones PH. Hes6 Is Required for the Neurogenic Activity of Neurogenin and NeuroD ［J］. PLoS One, 2011, 6(11): e27880.
/
| 〈 |
|
〉 |
