Aging related autofluorescence can be used as a marker of cell senescence

ZHANG Jing  YANG Lu-meng  CHEN Xiao-chun*

doi:10.3969/j.issn.0529-1356.2014.01.001

Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (1) : 1-6. DOI: 10.3969/j.issn.0529-1356.2014.01.001

Aging related autofluorescence can be used as a marker of cell senescence

ZHANG Jing¹ YANG Lu-meng¹CHEN Xiao-chun ^1,2*

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Abstract

Objective To describe the emission spectra of autofluorescence derived from lipofuscin in aged brain sections and to investigate how aging related autofluorescence is used reasonably and abolished properly. Methods Three months old and 24 months old C57BL/6J male and female mice were used in this study. Staining of senescenceassociated beta galatosidase and accumulation of lipofuscin were used to evaluate the degree of brain aging. Laser confocal microscopy was used to analyze the emission spectra of autofluorescence. Immunohistochemistry and immunofluorescence was used to identify astrocyte. Results Strong SA-β-Gal staining and autofluorescence and accumulation of lipofuscin appeared in the CA3 of hippocampus and the periventricular area of the third ventricle in 24 months old mice. The emission wavelength of aging related autofluorescence was between 500-565 nm. Astrocytes in periventricular area of the third ventricle were of not only strong SA-β-Gal staining, but also strong autofluorescence. Abolition of background autofluorescence from lipofuscin following immersion in autofluorescence eliminator reagent was observed in aged brain tissue. Conclusion Aging related autofluorescence can be used as a marker of cell senescence with a specific phenotype. Reduction of background autofluorescence from lipofuscin can improve the specificity of immunofluorescence detection in aged brain tissue samples.

Key words

Senescence / Brain / Lipofuscin / Autofluorescence / Immunofluorescence / Mouse

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ZHANG Jing YANG Lu-meng CHEN Xiao-chun*. Aging related autofluorescence can be used as a marker of cell senescence[J]. Acta Anatomica Sinica. 2014, 45(1): 1-6 https://doi.org/10.3969/j.issn.0529-1356.2014.01.001

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