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Isolation and culture of first trimester human trophoblast cell
ZUO Yan-zhen ZHANG Kong-yan LI Yu-hong* XU Qian MAO Xiao-dan TAN Yu-si
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (6) : 861-864.
Isolation and culture of first trimester human trophoblast cell
Objective To research a simple method for purification of first trimester human trophoblast cell in vitro.Methods Whole first trimester human chorionic tissues were isolated with trypsin/collagenase. HE staining and immunocytochemistry methods were used to detect the tyophoblast cells purity. Results Cytokeratin-7 positive cells were more than 90%. Conclusion First trimester human Trophoblast cells can be obtained through the simple method and may offer enough cells for later experiments.
Tyophoblast cell / First trimester / Primary cell culture / Percoll gradient / Human
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