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Effects of extract of notopterygium on Th1/Th2 cells balance and p38 signaling pathway in the asthma mouse model
LI Liang-chang PIAO Hong-mei QIN xiang-zheng YAN Guang-hai LI Guang-zhao*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (6) : 819-823.
Effects of extract of notopterygium on Th1/Th2 cells balance and p38 signaling pathway in the asthma mouse model
Objective To explore the effect of extract of notopterygium (EN) on Th1/Th2 cells balance and signaling pathway p38 in the asthma mouse model. Methods Sixty male BABL/c mice were randomly divided into six groups with 10 mice per group: normal group, ovalbumin（OVA）group, notopterygium low dose group, notopterygium high dose group, SB203580 group and dexamethasone group. The left lung was isolated for pathological examination. Lung sections were stained with HE. The number of total cells and different inflammatory cells were counted by an optical microscope. The concentrations of interleukin(IL)-4，IL-5，IL-13 and interferon-γ(IFN-γ) in bronchoalveolar lavage fluid (BALF) were measured by ELISA. The Western blotting was performed to detect the IL-4, IFN-γ， P38 in lung tissue. Results In OVA group, the number of total cells and inflammatory cells, the concentrations of IL-4, IL-5, IL-13 in BALF were significantly higher than those in normal group (P<0.05), but the concentration of IFN-γ in BALF was significantly lower than in normal group(P<0.05). Pulmonary inflammatory infiltration was more serious in OVA group than in normal group(P<0.05). IL-4 and phosphorylated P38 in lung tissue were significantly higher in OVA group than those in normal group (P<0.05), but IFN-γ level of lung tissue was significantly lower than in normal group (P<0.05). In notopterygium low and high dose groups, SB20358 group and dexamethasone group, the number of total cells and inflammatory cells, the concentrations of IL-4, IL-5, IL-13 in BALF and IL-4, phosphorylated P38 in lung tissue were significantly lower than those in OVA group (P<0.05), the level of IFN-γ in BALF and lung tissue was significantly higher than in OVA group (P<0.05); Notopterygium, SB20358 and dexamethasone significantly reduced pulmonary inflammatory infiltration(P<0.05). Conclusion All these results indicate the extract of notopterygium roots can inhibit the development of asthma in the mouse model, and its possible mechanism is that Th1/Th2 cells balance may be changed while p38 signaling pathway is inhibited.
Asthma / Notopterygium / P38 mitogen-activated protein kinases / ELISA / Western blotting / Mouse
［1］ Sun Y, Xu Q. Aqueous extract from rhizoma notopterygii reduces contact sensitivity by inhibiting lymphocyte migration via down-regulating metalloproteinase activity［J］. Pharmacol Res, 2002, 46(4):333-337.
［2］Zhang X，Huang BY. The observation of curative effect on the treatment of acute gouty arthritis with notopterygii decoction［J］. Chinese Journal of Traditional Medical Traumatology & Orthopeics, 2000, 10(3):34-35.(in Chinese)
张贤，黄波禹. 羌活汤治疗痛风性关节炎疗效观察［J］. 中国中医药骨伤科杂志，2000，10（3）：34-35.
［3］Tang YX. The therapeutic effect of notopterygium on cardiovascular and cerebrovascular diseases［J］. China Journal of Chinese Materia Medica, 2001, 26(5):346-348. (in Chinese)
唐迎雪. 谈羌活对心脑血管疾病的治疗作用［J］. 中国中药杂志，2001，26（5）：346-348.
［4］Li LCh, Qin XZh, Li GZh, et al. Effects of anthocyanin-rich extract from black rice on p38 mitogen-activated protein kinases signaling pathway in asthmatic mice［J］. Acta Anatomica Sinica, 2012，43(1):51-54. (in Chinese)
李良昌，秦向征，李光昭，等. 黑米花色苷提取物在小鼠哮喘模型中队p38丝裂原活化蛋白激酶信号通路的影响［J］. 解剖学报，2012,43（1）：51-54.
［5］Tournoy KG., Kips JC, Schou C, et al. Airway eosinophilia is not a requirement for allergen-induced airway hyperresponsiveness［J］. Clin Exp Allergy,2000, 30(1):79-85.
［6］Larche M, Robinson DS, Kay AB. The role of T lymphocytes in the pathogenesis of asthma［J］. J Allergy Clin Immunol, 2003,111(3):450-463.
［7］Kampen GT, Stafford S, Adachi T, et al. Eotaxin induces degranulation and chemotaxis of eosinophils through the activation of ERK2 and p38 mitogen-activated protein kinases［J］. Blood, 2000, 95(6):1911-1917.
［8］Chen CH, Zhang DH, LaPorte JM, et al. Cyclic AMP activates p38 mitogen-activated protein kinase in Th2 cells: phosphorylation of GATA-3 and stimulation of Th2 cytokine gene expression［J］. J Immunol, 2000,165(10):5597-5605.
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