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Changes of vimentin and matrix metalloproteinases-2 expressions in epidermal grouth factor induced migration of human umbilical vein endothelial cells
LU Shi-jun YANG Hui-ke MA Wen-ping LI Xiao-dong ZHANG Ya-fang*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (6) : 789-794.
Changes of vimentin and matrix metalloproteinases-2 expressions in epidermal grouth factor induced migration of human umbilical vein endothelial cells
Objective To evaluate the relationship between vimentin and migration induced by epidermal growth factor (EGF) in human umbilical vein endothelial cells (HUVECs), the expressions of vimentin and matrix metalloproteinases-2 (MMP-2) were examined in epidermal growth factor (EGF) -induced migration of HUVECs. Methods In the current study, the migration of HUVECs was analyzed after EGF stimulation for 10h by scratch assay, and the effect of EGF on the cells morphology was also observed. The monolayer HUVECs were scratched to induce the cells migration in both control group and EGF group, and then immunofluorescent staining was used to detect the vimentin expression. Both vimentin and MMP-2 expressions were assessed via Western blotting and RT-PCR. Results The scratch assay revealed a 1.8-fold increase induced by EGF in migration levels of HUVECs. Interestingly, the HUVECs morphology also altered dramatically from cobblestone-like shape to spindle-like shape after EGF stimulation. As a result of EGF stimulation, both vimentin and MMP-2 expressions in HUVECs were up regulated in both protein and mRNA levels (P<0.05). Conclusion EGF upregulates both vimentin and MMP-2 expressions effectively in EGF-induced migration of HUVECs, and vimentin is closely associated with the migration in HUVECs.
Epidermal growth factor / Migration / Vimentin / Matrix metalloproteinase 2 / Western blotting
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