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Changes of RUNX1T1 mRNA and protein expression in rat hippocampus after fimbria/fornix transection
ZOU Lin-qing JIN Guo-hua* WANG Hai-qin LI Hao-ming ZHU Pei-pei QIN Jian-bing
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (6) : 767-771.
Changes of RUNX1T1 mRNA and protein expression in rat hippocampus after fimbria/fornix transection
Objective To observe the temporal and spatial changes of RUNX1T1 mRNA and protein expression in rat hippocampus after fimbria/fornix transection at different time points. Methods Rat models were prepared by cutting fimbria fornix, and divided into normal group, cutting 3days, 7days, 14days, 21days and 28days groups. 1. The total hippocampal tissue mRNA was extracted, and the expression of RUNX1T1 mRNA was detected by real-time PCR; 2. The total protein of hippocampus was extracted, and the expression of RUNX1T1 protein was detected by western blotting; 3. Frozen brain sections were detected by RUNX1T1 immunofluorescence and Hoechst labeled, to observe the RUNX1T1/Hoechst double labeled positive cells in hippocampal dentate gyrus. Results Real-time PCR analysis showed that the RUNX1T1 mRNA expression in the hippocampus was significantly upregulated on the 3rd day after transection. Western blotting analysis indicated that the expression of RUNX1T1 protein was detected in the hippocampus of rats at basal condation, and the levels of RUNX1T1 protein were markedly increased at days 3 and 7, and peaked at day 3 after transection. In contrast, no siginificant change in the RUNX1T1 protein expression at days 14, 21 and 28. Immunostaining showed that the nember of RUNX1T1-positive cells was siginificantly higher at day 3 compared with control and decreased slowly after day 7. Conclusion The transient upregulation of RUNX1T1 mRNA and protein expression in the early phase after fimbria/fornix transection, and location in the hippocampal dentate gyrus granule lower, which may be related to the hippocampal neural regeneration.
RUNX1T1 / Hippocampus / Fimbria/fornix transection / Neural regeneration / Immunofluorescence / Rat
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