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Activated astrocyte secrete clusterin that enhances neuronal differentiation from rat hippocampal neural stem cells
ZOU Lin-qing JIN Guo-hua* LI Hao-ming QIN Jian-bing TIAN Mei-ling ZHU Pei-pei WANG Hai-qin
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (6) : 761-766.
Activated astrocyte secrete clusterin that enhances neuronal differentiation from rat hippocampal neural stem cells
Objective To detect the secretion of clusterin in "reactive astrocytes" which are activated by fimbria-fornix transected hippocampal extracts, and to investigate the neuronal differentaition of hippocampal neural stem cells (NSCs) by clusterinin vitro. Methods The astrocytes were cultured in fimbria-fornix transected hippocampal extracts, normal hippocampal extracts and DMEM/F12 medium, the conditional medium contained normal hippocampal extracts and transected 12hours, 24hours, 48hours hippocampal extracts. The concentrations of clusterin was detected by ELISA assay. To investigate the neuronal differentaition of hippocampal neural stem cells (NSCs) by clusterin, the cells were divided into the induced group and control group. Seven days later, the neuronal differentiation of hippocampal NSCs was investigated by MAP-2 immunofluorescence and labeled by Hoechst. Results The concentrations of clusterin in normal group and control group were lower than the transected 12hours, 24hours, 48hours groups, especially the 24hours group which concentration was about 4-folds higher than normal and control group，about (0.1037±0.0119)ng/L,(0.1170±0.0078) ng/L, (0.1940±0.0364) ng/L, (0.4250±0.0724) ng/L and (0.2903±0.0472) ng/L, respectively. The number of MAP-2 positive cells in clusterin induced group was higher than the control group (P<0.05), and the percentages of the total number of cells of these two groups were (11.67± 2.57)% and (4.52±1.54)%, respectively. The processes in the induced group was longer and more than the control group. Conclusion Reactive astrocytes activated by fimbria-fornix transected hippocampal extracts secrete more clusterin. The clusterin in culture cells may promote hippocampal NSCs differentiation into mature neurons in vitro.
Astrtocyte / Clusterin / Hippocampus / Neural stem cell / Neuron / Immunofluorescence / Rat
［1］Zhang X, Jin G, Tian M, et al. The denervated hippocampus provides proper microenvironment for the survival and differentiation of neural progenitors［J］. Neurosci Lett, 2007,414(2):115-120.
［2］ Zou LQ, Jin GH, Dong ChM, et al. Proliferation and diferentiation of the endogenous neural stem cells in hippocampus into neurons after fimbria fornix transection［J］. Suzhou University Journal of Medical Science, 2007,27(5):681-683.(in Chinese)
邹琳清, 金国华, 董传明, 等. 穹隆海马伞切割后海马内NSCs的增殖和向神经元的分化［J］. 苏州大学学报(医学版), 2007,27(5):681-683.
［3］Zou L, Jin G, Zhang X, et al. Proliferation, migration and neuronal differentiation of the endogenous NSCs in hippocampus after fimbria fornix transection［J］. Int J Neurosci, 2010,120(3): 192-200.
［4］Jin GH，Zhang XH，Tian ML, et al. Promoting effect of the extracts from hippocampus in fimbria fornix transection side on the neural stem cell differention into neurons［J］. Acta Anatomica Sinica, 2004,35(2):137-141. (in Chinese)
金国华, 张新化, 田美玲, 等. 穹隆海马伞切割侧海马提取液对神经干细胞分化为神经元的促进作用［J］. 解剖学报, 2004,35(2):137-141.
［5］ Xu LL,Wang L,Yi X, et al. The change of BLBP expression in rat hippocampus after the fimbria/fornix transection［J］. Chinese Journal of Neuroanatomy,2008,24(6):570-574. (in Chinese)
徐璐璐, 王磊, 衣昕, 等.切割穹隆海马伞大鼠海马内BLBP的表达变化［J］. 神经解剖学杂志, 2008,24(6):570-574.
［6］Zhao HY, Tian ML,Shi JH, et al. The induced activation and significance of hippocampal radial glial cells in vitro［J］. Chinese Journal of Neuroanatomy,2009,25(4):375-380. (in Chinese)
赵荷艳, 田美玲, 施金洪, 等. 海马放射状胶质细胞的体外诱导激活及其意义［J］. 神经解剖学杂志, 2009,25(4):375-380.
［7］Zhao H, Jin G, Tian M, et al. Extract of deafferented hippocampus promotes in vitro radial glial cell differentiation into neurons［J］. Neurosci Lett, 2011,498(1):93-98.
［8］Zhou W,Guan Q, Kwan CC, et al. Loss of clusterin expression worsens renal ischemia-reperfusion injury［J］. Am J Physiol Renal Physiol, 2010, 298(3):568-578
［9］Kim N, Choi WS. Proapoptotic role of nuclear clusterin in brain［J］. Anat Cell Biol, 2011,44(3):169-175.
［10］Yang WW，Jin GH，Shi JH, et al. The synergetic effect of HCNP and hippocampal extracts on neural stem cells differentiation to cholinergic neurons［J］. Chinese Journal of Neuroanatomy,2011,27(5):539-544. (in Chinese)
杨伟伟,金国华,施金洪，等. HCNP 与海马提取液对神经干细胞向胆碱能神经元分化的协同作用［J］. 神经解剖学杂志，2011,27(5):539-544.
［11］Li L, Lundkvist A, Andersson D, et al. Protective role of reactive astrocytes in brain ischemia［J］. J Cereb Blood Flow MeTab, 2008, 28(3):468-481.
［12］Jha MK, Seo M, Kim JH, et al. The secretome signature of reactive glial cells and its pathological implications［J］. Biochim Biophys Acta, 2013, 1834(11):2418-2428.
［13］Charnay Y, Imhof A, Vallet PG,et al. Clusterin expression during fetal and postnatal CNS development in mouse［J］. Neuroscience,2008,155(3):714-724.
［14］Li S, Guan Q, Chen Z, et al. Reduction of cold ischemia-reperfusion injury by graft-expressing clusterin in heart transplantation［J］. J Heart Lung Transplant, 2011,30(7):819-826.
［15］Yamashita S, Tai P, Charron J,et al. The leydig cell MEK/ERK pathway is critical for maintaining a functional population of adult leydig cells and for fertility［J］. Mol Endocrinol, 2011, 25(7): 1211-1222
［16］Al-Asaaed S, Winquist E. Custirsen (OGX-011): clusterin inhibitor in metastatic prostate cancer［J］. Curr Oncol Rep, 2013,15(2):113-118
［17］Guan Q, Li S, Yip G, et al. Decrease in donor heart injury by recombinant clusterin protein in cold preservation with University of Wisconsin solution［J］. Surgery, 2012,151(3):364-371.
［18］Wang Y, Wang X, Zhao H, et al. Clusterin confers resistance to TNF-alpha-induced apoptosis in breast cancer cells through NF-kappaB activation and Bcl-2 overexpression［J］. J Chemother, 2012,24(6):348-357.
［19］Cordero-Llana1 O, Scott SA, Maslen SL, et al. Clusterin secreted by astrocytes enhances neuronal differentiation from human neural precursor cells［J］. Cell Death Differ, 2011,18(5): 907-913.
［20］Wyatt A, Yerbury J, Poon S, et al. Chapter 6: the chaperone action of clusterin and its putative role in quality control of extracellular protein folding［J］. Adv Cancer Res, 2009,104:89-114.
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