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Interaction between cell migration and vasculature in the developing cerebellum of the mouse
CHEN Wen-jing ZHANG Wen-ling LI Xue WANG Qiang LIU Bin DENG Jin-bo*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (6) : 740-747.
Interaction between cell migration and vasculature in the developing cerebellum of the mouse
Objective Our aim was to explore the interaction between angiogenesis and neural migration in the developing cerebellum of mice. Methods The immunofluorescence, BrdU and ink perfusion were used to label the radial glial cells, Purkinje cells, granule cells and vasculature in the mouse cerebellum aged from E10 (embryonic day 10) to P90 (postnatal day 90). A total of 146 mice at various ages were used in the study. Results Vascular network began to appear in the cerebellum at about E15. Tith age increasing, vascular density increased. In the meantime, there was close relationship between the radial glial cell development and vascular development. For instance, blood vessels and the projections of radial glial cells in the external granular layer were arranged regularly and paralleled each other, but the distributions of vessels and radial glial fibers in the internal granular layer and white matter were in disorder, suggesting the interaction between the vasculature and radial glial cells. Finally, the study also showed that many BrdU-positive cells migrated along blood vessels. Conclusion Cell migration plays a crucial role in cerebellar lamellae formation process. We found that the blood vessels not only have interaction with the radial glial cells, but also guide the migration of neurons in the cerebellum, probably serving as scaffolds for the neuronal migration.
Cerebellum / Neural cell / Vessel / Cell migratio / Immunofluorescence / Mouse
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