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Effects of maturation in vitro on mitochondrial DNA copy and functions in oocytes
GE Hong-shan* LI Xiao-he ZHANG Fan CHEN Hua XI Hai-tao HUANG Jian-ying ZHU Chun-fang Lü Jie-qiang
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (5) : 680-684.
Effects of maturation in vitro on mitochondrial DNA copy and functions in oocytes
Objectiv To determine whether in vitromaturation (IVM) has an impact on oocyte’s mitochondrial number, and functions, and on oocyte’s quality.
Methods The maturation mice oocytes were collected following either in vitro or in vivo (IVO) maturation process. The copies of mtDNA, the magnitude of mitochondrial membrane potential (ΔΨm) and oocyte adenosine triphosphate (ATP) content, pattern of mitochondrial distribution, reactive oxygen species（ROS）level, and the integrity of spindle and chromosome alignment were evaluated by RT-PCR, immunofluorescence and fluorescence-luciferase mensuration. Results Significant differences were detected in the copies of mtDNA，ROS level and integrity of cytoskeleton in oocytes between IVM and IVO.There were no significant differences in mitochondrial distribution, oxidative phosphorylation function in oocyte between IVM and IVO. Conclusion The in vitro non-physiological maturation may inhibit mtDNA replication, and increase ROS production and rates of abnormal spindle and chromosome alignment. This may partly explain the low development potential of IVM oocyte.
Oocyte /
Mitochondria /
Rective oxygen species /
Cytoskeleton /
In vitromaturation /
Real-time PCR /
Immunofluorescence /
Mouse
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