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Influence of annexin A7 low expression on proliferation of HepG2 cells
WANG Xiao-jie LI Xin*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (5) : 656-660.
Influence of annexin A7 low expression on proliferation of HepG2 cells
Objective To study whether annexin A7(ANXA7) low expression can affect proliferation of HepG2 cells. Methods Western blotting was used to identify siRNA can highly surpress annexin A7 expression. When the effect of the siRNA was assured, the siRNA was transfected into HepG2 cells by lipofectamine transfection method. At the same time, cells in negative control group were transfected negative siRNA while cells in blank control group only added lipofectamine 2000. Twenty-four, forty-eight and seventy-two hours after transfection ,cell counting was used to draw growth curve. Forty-eight hours after transfection, MTT assay was used to detect proliferation activity; Immunohistochemistry was applied to observe the expression of cyclinD1 and Ki67; Western blotting was performed to detect the expression of cyclinD1. Results Cell counting and MTT assay showed that proliferation of the siRNA interference group decreased significantly(P<0.05). The expression of cyclinD1 and Ki67 decreased compared with the negative control group and the blank control group(P<0.05). Conclusion ANXA7 low expression may inhibit proliferation of HepG2 cells.
ANXA7 / HepG2 cell / cyclinD1 / Ki67 / Immunohistochemistry / Western blotting / Human
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