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Localization and clinical significance of kindlin-1 in human gingival epithelia
XIE Ting-ting MEI Fang ZHONG Jin-sheng TANG Yan ZHANG Hong-quan*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (5) : 641-645.
Localization and clinical significance of kindlin-1 in human gingival epithelia
Objectiv To investigate distributions of kindlin-1 in human normal gingival epithelia and chronic inflammatory gingival epithelia and establish clinical relevance between kindlin-1 and periodontal diseases.Methods Immunohistochemistry and Western blotting techniques were applied to detect the localization and expression of kindlin-1 of 10 human normal gingival epithelia and 8 chronic inflammatory gingival epithelia. Results Kindlin-1 was mainly localized in basal layer cells and upper granular layer cells of normal gingival epithelia, especially highly expressed in cytoplasm in granular layer cells, with some diffuse staining in the nuclei of suprabasal layer cells. In addition, the higher immunoractivity for kindlin-1 was observed in interdental gingiva than in free marginal gingiva (P<0.05). Compared to normal gingival epithelia, kindlin-1 presented much wider and higher expression in chronic inflammatory gingival epithelia (P<0.05). Western blotting further verified that kindlin-1 in chronic inflammatory gingival epithelia was higher expressed than in normal gingival epithelia (P<0.05). Conclusion The localization and expression of kindlin-1 in human gingival epithelia are closely associated with the differentiation and inflammation.
Kindlin-1 / Gingival epithelia / Immunohistochemistry / Western blotting / Human
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