Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Effect of Xing-naojing and Shengmai injection on protease activated receptor-1 expression in rats after intracerebral hemorrhage
ZHANG Qing JIANG Yan-xia LI Guo-liang WANG Qi-xin MA Cheng-tai* WANG Shou-biao YAO Ru-yong
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (5) : 630-634.
Effect of Xing-naojing and Shengmai injection on protease activated receptor-1 expression in rats after intracerebral hemorrhage
Objective To investigate the effect of Xing-naojing and Shengmai injection on protease activated receptor-1(PAR1) expression in rat brain tissue after intracerebral hemorrhage (ICH).Methods The ICH model was induced by injecting blood of its own. Forty male SD rats were randomly divided into ICH group (n=10), saline group (NS, n=10), Xing-naojing and Shengmai injection group (XNJSM, n=10), hirudin group (HIR, n=10). Hematoxylin and eosin staining was performed to examine perihematomal neurocyte morphological changes. The expression of PAR1 in perihematomal tissue was examined by immunohistochemistry and Western blotting techniques. Results The expression of PAR1 in perihematomal tissue was increased after ICH. However,compared with ICH group, perihematomal tissue morphological changes were improved obviously. The expression of PAR1 was decreased significantly between XNJSM group and HIR group(P<0.05). Conclusion XNJSM injection may play a neuroprotective effect by inhibiting the expression of PAR1 protein after ICH.
Intracerebral hemorrhage / Xing-naojing and Shengmai injection / Protease-activated receptor-1 / Immunohistochemistry / Western blotting / Rat
［1］ Katsuki H. Exploring neuroprotective drug therapies for intracerebral hemorrhage［J］.J Pharmacol Sci, 2010,114(4):366-378.
［2］Nakamura T, Xi G, Park JK, et al. Holo-transferrin and thrombin can interact to cause brain damage［J］. Stroke, 2005,36(2):348-352.
［3］Xi G, Keep RF, Hoff JT. Mechanisms of brain injury after intracerebral hemorrhage［J］. Lancet Neurol, 2006,5(1):53-63.
［4］Ma ChT. The experience of syndrome differentiation and treatment cerebral hemorrhage at acute phase by professor Wang［J］. Sichuan Traditional Chinese Medicine, 2001,19(2):5-6. (in Chinese)
马承泰. 王左教授辨治脑出血急性期经验［J］. 四川中医，2001,19(2):5-6.
［5］Zhou ZhH, Qu F, He X, et al. An improved autologous blood ICH model of rat:double injection and double withdrawal［J］.Chinese Journal of Clinical Neurosciences, 2004,12(4):406-408. (in Chinese)
周中和, 曲方, 何祥, 等. 一种改良大鼠自体血脑出血模型：二次注血/退针法［J］. 中国临床神经科学，2004,12(4):406-408.
［6］Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats［J］. Stroke,1989, 20(1):84-91.
［7］Figueroa BE, Keep RF, Betz AK, et al. Plasminogen activators potentiate thrombin-induced brain injury［J］. Stroke, 1998,29(6):1202-1208.
［8］Xi G, Wagner R, Keep RF, et al. Role of blood clot formation on early edema development following experimental intracerebral hemorrhage［J］. Stroke, 1998,29(12):2580-2586.［9］Coughlin SR. Thrombin signalling and protease-activated receptors［J］. Nature, 2000, 407(6801): 258-264.
［10］Tulinsy A. Molecular interactions of thrombin［J］. Sem in Thromb Hemost, 1996,22(2):117-124.
［11］Fujimoto S, Katsuki H, Kume T, et al. Thrombin-induced delayed injury involves multiple and distinct signaling pathways in the cerebral cortex and the striatum in organotypic slice cultures［J］. Neurobiol Dis, 2006,22(1):130-142.
［12］Ohnishi M, Katsuki H, Fujimoto S, et al. Involvement of thrombin and mitogen-activated protein kinase pathways in hemorrhagic brain injury［J］. Exp Neurol, 2007,206(1):43-52.
［13］Ardizzone TD, Zhan X, Ander BP, et al. SRC kinase inhibition improves acute outcomes after experimental intracerebral hemorrhage［J］. Stroke, 2007,38(5):1621-1625.
［14］Hamill CE, Mannaioni G, Traynelis SF,et al. Protease-activated receptor1-dependent neuronal damage involves NMDA receptor function［J］. Exp Neurol, 2009,217(1):136-146.
［15］Vandell AG, Larson N, Laxmikanthan G, et al. Protease-activated receptor dependent and independent signaling by kallikreins 1 and 6 in CNS neuron and astroglial cell lines［J］. J Neurochem, 2008,107(3):855-870.
［16］Junge CE, Lee CJ, Traynelis SF,et al. Protease-activated receptor-1 in human brain: localization and functional expression in astrocytes［J］.Exp Neurol,2004,188(1):94-103.
［17］Wang Y, Richter-Landsberg C, Reiser G. Expression of protease-activated receptors (PARs) in OLN-93 oligodendroglial cells and mechanism of PAR-1-induced calcium signaling［J］. Neuroscience, 2004, 126(1):69-82.
［18］Suo Z, Wu M, Ameenuddin S, et al. Participation of protease-activated receptor-1 in thrombin-induced microglial activation［J］. J Neurochem, 2002,80(4):655-666.
［19］Bartha K, Domotor E, Lanza F, et al. Identification of thrombin receptors in rat brain capillary endothelial cells［J］. J Cereb Blood Flow Metab, 2000, 20(1):175-182.
［20］Ma ChT, Jiang YX, Wang Z. Experimental study of the protect effect of Xingnaojing-shengmai decoction on the cortical astrocytes injurying by thrombin［J］. Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care, 2003,10(1):46-48. (in Chinese)
马承泰，蒋艳霞，王左.醒脑静生脉混合液对凝血酶损伤星形胶质细胞的保护作用［J］. 中国中西医结合急救杂志, 2003,10(1):46-48.
/
| 〈 |
|
〉 |
