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Effect of knocking down N-cadherin expression on the tectum neuronal migration and neurofilament expression during chick embryo development
YANG Ci-qing LI Qiong FU Su-lei LI Han FAN Yi-feng GUO Zhi-kun LIN Jun-tang*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (5) : 612-615.
Effect of knocking down N-cadherin expression on the tectum neuronal migration and neurofilament expression during chick embryo development
Objective To clarify the role and function of the N-cadherin and its impact on the expression of neurofilaments in central nervous system during the chick embryo development. Methods Fresh eggs were cultured in normal condition for three days (E3), and 3-4ml of egg white was removed from egg, then continuing culture to E5. N-cadherin interference plasmids (pGPU6-GFP-neo-N-cadherin-shRNA) was injected into the optic tectum and then handled by electroporationin vivotechnology. Tissues were obtained at E12 and from each group three embryos were collected, fixed with 4%PFA, embeded with OCT, and cut into frozen sections. Fluorescence immunohistochemistry method was used to analyses the different protein expression. Results Expression of N-cadherin was inhibited, which caused neuronal migration disorder. The expression of NF was also suppressed. Conclusion Inhibition of N-cadherin expression can lead to neuronal migration disorder, affecting the expression of NF.
Embryonic development / Optic tectum / Neural cadherin / Neurofilament / Electroporationin vivo / Chick embryo
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