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Electrophysiological characteristics of cultured hippocampal cholecystokinin positive neurons
ZHANG Yang-hui WEI Xiao-fei* SI Jun-qiang MA Ke-tao ZHANG Lu-lu WANG Qin-wen
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (5) : 607-611.
Electrophysiological characteristics of cultured hippocampal cholecystokinin positive neurons
Objective To explore the electrophysiological properties of cultured hippocampal cholecystokinin (CCK) positive (CCK+ ) neurons. Methods The cultured hippocampal CCK+ neurons were labeled by an adenovirus encoding CCK promoter and green fluorescent protein (GFP) and then whole cell patch clamp technique was carried on to explore the electrophysiology characteristic of hippocampal CCK+ neurons. Results The long diameter of CCK+ neurons’cell body was (22.85±0.77)μm, and the short diameter was (16.21±0.42)μm. The membrane electrophysiology parameters of CCK+ neurons were as following.
Rest membrane potential was(-55.90±1.30)mV. Membrane capacity was (45.77±2.06)pF. Membrane resistance was (711.00±46.69)MΩ. The action potential firing frequency of cultured hippocampal CCK+ neurons was (26.17±3.41)Hz. The spontaneous inhibitory postsynaptic currents frequency of CCK+ neurons was (5.26±0.71)Hz, which was significant higher compared to the frequency of spontaneous excitatory postsynaptic currents ［(3.24±0.62)Hz］. Conclusion The electrophysiology characteristics of hippocampal CCK+ neuron may be colsely related to its modulatory effect in central nervous system.
Cholecystokinin / Interneuron / Hippocampus / Whole cell patch clamp / Rat
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