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Improvement of Masson staining in renal fibrosis of mice
WEI Sheng-nan ZENG Xiang-jun LI Hui-hua*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (4) : 576-579.
Improvement of Masson staining in renal fibrosis of mice
Objective To get an improved Masson 3 staining method for staining renal fibrosis. Methods Bouin‘s fluid was used to re-fix the paraffin fixed sections. Ethanol∶acetic acid（2∶1) which was stored at -20℃ was used to treat the sections;
Brilliant green was used to substitute aniline blue. Duration of brilliant green staining was decreased. Results Renal interstitium of fibrosis and non-fibrosis were distinguished. Collagen fibers appeared green (counter staining by brilliant green) and cytoplasm
and muscle fibers were red. Conclusion In improved Masson 3 staining, tissues of fibrosis and non-fibrosis are distinguished by brilliant green, the staining results are stable and uniform. The method demonstrates a satisfied compared staining result.
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