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Expression of thyroid hormone receptors in the heart of neonatus rats with maternal hyperthyroidism
CHEN Jing* LI Bin SONG Fang FENG Ruo-peng ZHAO Zi-wei
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (4) : 546-549.
Expression of thyroid hormone receptors in the heart of neonatus rats with maternal hyperthyroidism
Objective To detect the expression of thyroid hormone receptors(TR), including TRα1,TRα2 and TRβ1. Methods The animal model of hyperthyroidism was established (population and quantity:24 female Wistar rats). Took the neonatal rat hearts
of day 5, day 10 and day 15 after birth and stained paraffin sections with Masson staining. Detected expression of thyroid hormone receptors including TRα1,TRα2 and TRβ1 by real-time PCR. Results In the cardiac tissue, the TRα1 expression intensities were
higher than TRa2 and TRβ1. In addition, there was a peak in day 10 of neonatal rats both in the hyperthyroidism and the normal ones. In the hyperthyroidism rat, the TRα1 expression intensities in day 5 and day 10 increased significantly,while it decreased
in day 15 in hyperthyroidism. There was no significant difference of TRa2 expression in day 5, day 10 and day 15 between hyperthyroidism and normal. The TRβ1 expression intensity decreased in day 5 in hyperthyroidsm, however, it increased in day 10. Conclusions
The maternal hyperthyroidism may influence the cardiac muscle of the neonatal rats by the different expression of thyroid hormone receptors. Due to the significant change of TRα1, it may play a very important role in myocardial damage of hyperthyroidism.
Maternal hyperthyroidism / Thyroid hormone receptor (TR) / Real-time PCR / Neonatal rat
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