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Expression of integrin CD11a, CD11b and CD11c during the rat heart development
MU Ling-min WANG Wen-feng GUO ZHi-kun* ZHANG Guang-mou
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (4) : 525-529.
Expression of integrin CD11a, CD11b and CD11c during the rat heart development
Objective To study the expression changes of integrins CD11a, CD11b and CD11c during the rat heart development.Methods By using RT-PCR and immunohistochemistry, the genes and the protein expression of CD11a, CD11b and CD11c of rat cardiac
tissue were detected on embryonic day 18(E18d), postnatal 5 days(P5d), postnatal 19 days(P19d), postnatal 40 days(P40d) and postnatal 1 year(P1y). Results Immunohistochemical results showed that CD11a, CD11b and CD11c expressed in cell plasma of rat
heart tissue, and the level of their expression was gradually decreased from E18d to P1y. RT-PCR results showed that the expressions of CD11a, CD11b and CD11c between each group were positive. Among them, the changes of CD11a transcripts were not
significant compared with that of P40d and P19d (P>0.05), and there were significant changes among other groups (P<0.05). The expressions of CD11b on E18d, P5d, P19d and P40d were of no significant changes compared with each other respectively (P>0.05),
and there was statistical significant in the other groups. The expression of CD11c between groups was statistically significant (P<0.01). Conclusion The expression of CD11a, CD11b and CD11c exists a quantity change during the development of the rat myocardium,
and different structures of integrin molecules showed similar expression rules during the heart development, so they may be play an important role on regulation in the development of myocardial cells.
Heart / Integrin CD / Immunohistochemistry / RT-PCR / Rat
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