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Expression and clinical significance of trefoil factor 3 in patiens with papillary thyroid carcinoma
ZHANG Xiao-jun WU Jing-fang* HUANG Jing ZHANG Jing ZHANG Jian-hong LIU Jun-chao LIU Bo XUE Gang*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (4) : 503-508.
Expression and clinical significance of trefoil factor 3 in patiens with papillary thyroid carcinoma
Objective To investigate the expression and significance of trefoil factor 3(TFF3) in serum and tissue of patients with papillary thyroid carcinoma．Methods A total of 153 patients with thyroid tumor were divided into papillary thyroid carcinoma
(PTC) group(PTC,n=46),adenoma group(A,n=51),papillary hyperplasia group(PH,n=36) and normal group,(N,n=153, normal thyroid tissue of every specimen). Sixteen normal human were served as the normal group in ELISA.The expressions of TFF3 protein and mRNA in
surgical specimens were detected by immunohistochemistry(IHC) SP, in situ hibridazation (ISH) and ELISA method was used to determine the serum TFF3 level. Results 1. The positive profiless of TFF3 protein and mRNA were brown granules in cytoplasm. The positive
rate of TFF3 in 66 PTC group, 36 PH group, 51 A group and 153 N group were 92.42％, 47.22%,31.37％ and 25.40% respectively.There was a positive correlation between expression of TFF3 mRNA and protein,though a little lower of TFF3 mRNA(P<0.0001). The
integral absorbance (IA) of TFF3 protein and mRNA in PTC was higher those in than other three groups (P<0.01). 2. The high expression rate of TFF3 protein/mRNA in patients with thyroid carcinoma was correlated with the TNM stage and lymph node metastasis
(P<0.01). 3. The concentration of TFF3 in serum with thyroid carcinoma was the highest among the four groups（P<0.001）. Serum concentrations of TFF3 in surgical specimens positive group was obviously higher than that in the negative group (P<0.05). Conclusion
TFF3 protein/mRNA expresses strongly in PTC tissues. Detection of the serum TFF3 concentration may be a reference index in diagnosis of thyroid carcinoma.
Trefoil factor 3 / Papillary thyroid carcinoma / Immunohistochemistry / In situ hybridization / Enzymelinked immunoadsordent assay / Human
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