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Effects of RhoA/ROCKI signal pathway on the taxol-induced cell-cycle arrest of cervical cancer cells
WANG Wei-li WANG Ping* WANG Feng HUANG Shuai-shuai REN Yu
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (4) : 485-491.
Effects of RhoA/ROCKI signal pathway on the taxol-induced cell-cycle arrest of cervical cancer cells
Objective To explore the effects of RhoA/ROCKI signal pathway in taxol-induced cell cycle arrest of cervical cancer cells. Methods The MTT method was used to determine the minimal inhibition concentration of taxol in C33A cells. Cell cycle and expression of RhoA protein were assayed by flow cytometry. Expressions of ROCKI, cyclins and cyclin-dependent kinases (CDKs)were determined by Western blotting. Results After cells were treated with 1μmol/L of taxol, the cell number of G2/M phase and the expression of RhoA/ROCKI protein increased dramatically in a time-dependent manner. However, the expressions of CDK1,cyclin B1, A and D1 proteins were decreased markedly. Moreover, these effects were totally reversed when cells pretreated with the inhibitor of RhoA (C3 transferase), and partly reversed by the treatment of ROCKI inhibitor (Y-27632), although the significance still existed as compared with the control. No marked effects were observed on the expression of CDK2 in cells treated with taxol or RhoA/ROCKI inhibitors. Conclusion Taxolinduced G2/M arrest in C33A cells was related to the decreases of specific cyclins and CDK, which is tightly regulated by the increased RhoA activity, but ROCKI may only play the role partly in this process.
Cervical cancer / RhoA/ROCKI signal pathway / Cell cycle / Cyclin / Cyclin-dependent kinases / Flow cytometry / Western blotting / Human
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