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Expression and function of tyrosine kinase receptor KIT in mouse colonic mucosa epithelium
KANG Qian XUE Hong SUN Hai-mei LI Xiao-shuang ZHANG Guo-quan LIANG Yuan-jing* ZHOU De-shan*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (3) : 393-396.
Expression and function of tyrosine kinase receptor KIT in mouse colonic mucosa epithelium
Objective To investigate the expression and function of KIT in mouse colonic mucosa.Methods Western blotting and
RT-PCR techniques were used to detect the expression of c-kit gene and KIT in colonic mucosa in C57BL/6 mice (wild-type).
Immunofluorescence staining was used to examine the distribution of KIT-positive cells in colonic mucosa epithelium of C57BL/6
mice (wild-type) and N-ethyl-N-nitrosourea (ENU)-induced c-kit point mutant homozygous mice (Wads m/m).Intraperitoneal injection
of 5-bromo-2-deoxyuridin (BrdU, 30 mg/kg) was used to observe and compare the BrdU-positive cells in distal colonic mucosa epithelium
in wild-type and Wadsm/m mice, and to evaluate the effect of KIT on the renewal of colonic mucosa epithelium. Results The result of
RT-PCR indicated that c-kit gene was expressed in colonic mucosa.Western blotting showed that KIT was expressed in colonic mucosa.
Immunofluorescence staining showed that KIT-positive cells existed in the epithelium of colonic mucosa and were mainly located in
the lower part of crypts. BrdU incorporation indicated that the renewal of the epithelium of colonic mucosa in homozygous mice was
significantly slower than that in wild-type mice. Conclusion C-kit gene and KIT protein are wildely expressed in the colonic
mucosa epithelium and might be involved in the renewal of the colonic epithelium.
KIT / Colonic epithelial renewal / Colonic mucosa / Western blotting / Mouse
［1］Voytyuk O, Lennartsson J, Mogi A,et al.Src family kinases are involved in the differential signaling from two splice forms of c-Kit ［J］. J Biologi Chemis，2003，278(11):9159-9166.
［2］Ma F, Chen F, Chi Y, et al.Isolation of pancreatic progenitor cells with the surface marker of hematopoietic stem cells［J］. Int J Endocrinol, 2012， 2012 :948683.
［3］Woidacki K, Popovic M, Metz M, et al.Mast cells rescue implantation defects caused by c-kit deficiency［J］. Cell Death Dis, 2013， 4(1):e462.
［4］Morita-Fujimura Y, Tokitake Y, and Matsui Y. Heterogeneity of mouse primordial germ cells reflecting the distinct status of their differentiation, proliferation and apoptosis can be classified by the expression of cell surface proteins integrinα6 and c-kit［J］. Dev Growth Differ, 2009, 51(6): 567-583.
［5］Stokman G, Stroo I, Claessen N, et al. Stem cell factor expression after renal ischemia promotes tubular epithelial survival［J］. PloS One,2010, 5(12):e14386.
［6］Colitti M. Expression of putative stem cell markers related to developmental stage of sheep mammary glands［J］.Anat Histol Embryol, 2010,39(6):555-562.
［7］Ruan HB, Zhang N,Gao X,et al. Identification of a novel point mutation of mouse proto-oncogene c-kit through N-ethyl-N-nitrosourea mutagenesis［J］. Genetics, 2005, 169(2): 819-831.
［8］Mei F, Han J, Huang Y,et al. Plasticity of interstitial cells of Cajal: a study in the small intestine of adult guinea pigs［J］. Anat Rec(Hoboken), 2009, 292(7):985-993.
［9］Grimbaldeston MA, Chen CC, Piliponsky AM, et al.Mast cell-deficient W-sash c- kit mutant kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo［J］. Am J Pathol, 2005, 167(3):835-848.
［10］Yan KS, Chia LA, Li X, et al.The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations［J］. Proc Natl Acad Sci USA,2012,109(2):466-471.
［11］Rothenberg ME, Nusse Y, Kalisky T, et al.Identification of a Ckit+ colonic crypt base secretory cell that supports Lgr5+ stem cells in mice［J］. Gastroenterology, 2012,142(5):1195-1205.
［12］Barker N, van Es JH, Kuipers J,et al.Identification of stem cells in small intestine and colon by marker gene Lgr5［J］. Nature, 2007, 449(7165): 1003-1007.
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