Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Cell proliferation and synaptophysin expression in the developing mouse lens
LI Xue CHEN Wen-jing ZHOU Shu-fang ZHAO Jing-ya DENG Jin-bo*
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (3) : 318-323.
Cell proliferation and synaptophysin expression in the developing mouse lens
Objective To study cell proliferation and the expression of cell cycle protein (cyclin D1) and synaptophysin
(SYN) in mouse lens. Methods A total 150 mice were used in the study. HE staining and DAPI staining were performed out to
observe the general structure of the mouse lens. Proliferating cell nuclar antigen (PCNA) immunofluorescent staining and
5-bromodeoxyuridine (BrdU) assay were used to mark the proliferative cells in lens. The expression of cyclin D1 protein and
synaptophysin in the lens were investigated with cyclin D1 and synaptophysin immunofluorescent staining. Results At about E8,
the lens were evolved from the lens placode with single columnar epithelium. Then, the hollow lens bulb developed further
and was filled with numerous fibroblasts, which gradually formed lens. BrdU and PCNA-positive cells were mainly distributed
in the epithelium of anterior capsule. However, P10 afterward, BrdU positive cells disappeared, while PCNA continued to express
in the anterior epithelial cells. Cyclin D1-positive cells were mainly distributed in the epithelium of anterior capsule and
the equator of the lens in the embryonic days and early postnatal days. After P5, cyclinD1-positive cells were mainly located
in the pre-equatorial area, and only few cyclin D1 positive cells were found in the equator. Synaptophysin protein expressed
in the matrix and some cell bodies of equator during the development.Conclusion The cell proliferation plays an important role
during the lens development and lens repair. Cell cycle proteins, cyclin D1 is probably involved in regulating the cells’disappear
in the equator, therefore the lens’s transparency can be kept. Synaptophysin’s expression in lens has important function in
lens’s transparency, through its regulation to calcium flow in cell bodies and matrix of lens.
Lens / Cell proliferation / Cell cycle protein / Synaptophysin protein / Immunofluorescence / Mouse
［1］ Shi HH, Guan HJ. The influence of IOL design on posterior capsule opacification［J］.International Journal of Ophthalmology, 2004, 4(5): 882-886. (in Chinese)
石海红，管怀进. 人工晶状体的设计对后囊膜混浊的影响［J］. 国际眼科杂志，2004，4(5)：882-886.
［2］ Jia YQ, Sun HM. Development trend of the intraocular lens［J］. Chinese Journal of Ophthalmology, 1990,26(6): 323-325.
佳袁琴，孙惠敏.人工晶体的发展趋势［J］. 中华眼科杂志，1990，26(6)：323-325.
［3］Znsman I, Reifen R, Livni O. Role of apoptosis, proliferating cell nuclear antigen and p53 protein in chem ically induced colon cancer in rats fed corncob fiber treated with the fungus pleurotuso streatus ［J ］. A nticancer Res, 1997,17 (3c) : 2105-2113.
［4］ Zang JF, Niu YL, Demg JB, et al. Neocortical lamination and cell cycle in the mouse［J］. Acta Anatomica Sinica, 2012, 43(1):19-27. (in Chinese)
臧建峰，牛艳丽，邓锦波，等. 小鼠大脑新皮质片层化形成过程和细胞周期变化［J］. 解剖学报，2012，43(1)：19-27.
［5］ Yang Ch. Some research of synaptophysin［J］. Henan Medical Research, 2004, 13(1): 85-89. (in Chinese)
杨春.突触素的若干研究［J］. 河南医学研究，2004，13(1)：85-89.
［6］ Xia ZhX, Wu Y, Feng ZhZh, et al. Establishment of lens development model in Kunming mouse［J］. Journal of Clinical Ophthalmology, 2011, 19(5): 458-460. (in Chinese)
夏朝霞，吴艺，冯志贞，等. 小鼠晶状体连续发育模型的建立［J］. 临床眼科杂志，2011，19(5)：458-460.
［7］ Cai ZhM, Chen RH, Liang P, et al. Light Microscopic and electron microscopic observation on the developing course of lens capsule and lens epithelium in fetus［J］. Chinese Journal of Anatomy, 1993,16(2):95-98. (in Chinese)
蔡兆明，陈瑞华，梁平，等.胎儿晶状体囊及晶状体上皮发育过程的光、电镜观察［J］. 解剖学杂志，1993，16(2)：95-98.
［8］ Wang XJ, Yuan ShL, Huang RM. An Observation of the effect of tanshinone on cancer cell proliferation by Brdu and PCNA Labeling［J］. Journal of West China University of Medical Sciences, 1996, 27(4): 388-391. (in Chinese)
王修杰，袁淑兰，黄韧敏. BrdU和PCNA标记观察丹参酮对癌细胞增殖的影响［J］. 华西医科大学学报，1996，27(4)：388-391.
［9］ Yang ZhL, Li YG, Liu GL, et al. Expression of cyclin D1, cdk4, p16 and Rb in gallbladder carcinoma and its significance［J］. Chinese Journal of Hepatobiliary Surgery, 2001, 2(7):84-86. (in Chinese)
杨竹林，李永国，刘国利,等. 胆囊组织中cyclin D1，cdk4，p16，Rb 表达及意义［J］. 中华肝胆外科杂志，2001，2(7)：84-86.
［10］Coqueret O.Linking Cyclins to transcriptional control［J］.Gene，2002，299(1-2): 35-55.
［11］ Liu J，Li GY，Xu DCh, et al. Expression and significance of synaptophysin in the oculomotor nucleus in midbrain of fetus［J］. Chinese Journal of Neuroanatomy, 2011, 27(2): 198-202. (in Chinese)
刘靖，李国营，徐达传,等. 突触素在中脑动眼神经核发育中的表达及意义［J］. 神经解剖学杂志，2011，27(2)：198-202.
［12］Bitel CL, Nora I. Perrone-Bizzozero NI, Frederikse PH. HuB/C/D, nPTB, REST4, and miR-124 regulators of neuronal cell identity are also utilized in the lens［J］. Mol Vis,2010,16:2301-2316.
［13］ Yan HL, Yu XM. Ocular Physiology［M］. Beijing:People’s Medical Publishing House, 2001: 69-74. (in Chinese)
阎洪禄，于秀敏.眼生理学［M］. 北京：人民卫生出版社，2001：69-74.
［14］ Li WH, Kang ZF. Modern medicine and traditional medicine research on cataract and thinking［J］. International Journal of Ophthalmology, 2010, 10(2): 288-291. (in Chinese)
李伟华,亢泽峰. 现代医学与传统医学对白内障的研究进展与思考［J］.国际眼科杂志，2010，10(2)：288-291.
/
| 〈 |
|
〉 |
