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Correlation of draxin expression and neural crest migration in chick embryonic spinal cord at different developmental stage
SU Yu-hong* ZHANG San-bing CUI Hui-xian KANG Lin WANG Lei DU Juan YUAN Shi-jun
Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (3) : 304-307.
Correlation of draxin expression and neural crest migration in chick embryonic spinal cord at different developmental stage
Objective To examine the correlation of draxin expression and neural crest migration in chick embryonic spinal
cord at different developmental stages.Methods Ten chick embryos at different stages were used. In situ hybridization and
immunohistochemistry were used to observe the localization of draxin expression, the migration pathway of neural crest cell-the
spatial correlation of draxin expression and the neural crest migration. Living tissue incubation with draxin alkaline phosphatase
(draxin-AP) recombinant protein was used to check the binding ability of migrating neural crest with draxin protein. Results With
development, the draxin expression and neural crest migration had a very similar anterior-posterior gradient in chick embryonic
spinal cord. Draxin was expressed in the dorsal neural tube, roof plate and dorsal tip of dermomyotome which surrounded the
migration pathway of neural crest cells. We also found that portion of migrating neural crest cells bound to draxin-AP protein
directly. Conclusion Draxin is expressed in the tissues surrounding the neural crest migration pathway and portion of migrating
neural crest cells binds to draxin protein directly. We conclude therefore that draxin may be involved in the regulation of neural
crest cell migration in chick embryonic spinal cord.
Draxin / Spinal cord / Neural crest / Migration / In situ hybridization / Immumohistochemistry / Chick embryo
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