Effect of &nbsp;sox19b gene knockdown on the eyes development in zebrafish

doi:10.3969/j.issn.0529-1356.2013.02.023

Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (2) : 264-268. DOI: 10.3969/j.issn.0529-1356.2013.02.023

Effect of sox19b gene knockdown on the eyes development in zebrafish

GAO Ming YANG Yang CHEN Xue-ran SHI Wei LIN Yu-shuang, HAO Ai-jun*

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Abstract

Objective To explore the function of sox19b on the eye development in zebrafish by knockdown of sox19b. Methods To inhibit the expression of sox19b by microinjection of sox19b morpholinos（MO）and then observe the effect of the development of the eyes in zebrafish by RT-PCR and the whole-mount in situhybridization. Results The development of the eyes was abnormal after knockdown of sox19b, mainly showed small eyes or eye deficiency and the abnormal retina and lens(n =57/93). The expression of some important genes during the development of the eyes, such as rx3, pax2a and vsx2, was decreased apparently in the sox 19b morphant. Conclusion The transcription factor, sox19b is critical for the development of the eyes via the regulation of the important eye-field transcription factors,EFTEs in early zebrafish embryos.

Key words

Sox19b / Morpholinos / Development of the eyes / Eye-field transcription factor / In situ hybridization / RT-PCR / Zebrafish

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GAO Ming YANG Yang CHEN Xue-ran SHI Wei LIN Yu-shuang, HAO Ai-jun*. Effect of sox19b gene knockdown on the eyes development in zebrafish[J]. Acta Anatomica Sinica. 2013, 44(2): 264-268 https://doi.org/10.3969/j.issn.0529-1356.2013.02.023

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