Association of transcriptional factor GATA4 expression patterns with arterial pole development of embryonic mouse heart

CAI Yu-jin  JING Ya*  QIAO Ai-xiu  YANG Yan-ping   LI Hai-rong  CUI Hui-lin  ZHANG Tao

doi:10.3969/j.issn.0529-1356.2013.02.021

Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (2) : 253-258. DOI: 10.3969/j.issn.0529-1356.2013.02.021

Association of transcriptional factor GATA4 expression patterns with arterial pole development of embryonic mouse heart

CAI Yu-jin¹JING Ya ^1* QIAO Ai-xiu² YANG Yan-ping¹ LI Hai-rong¹ CUI Hui-lin¹ ZHANG Tao¹

Author information +

History +

Abstract

Objective To explore the relationship of the expression of transcriptional factor GATA4 in the heart-forming field and the foregut endoderm with the morphological development of the arterial pole of embryonic mouse heart.Methods Serial sections of mouse embryos on embryonic day(ED)7.5 to ED13, three embryos from each embryonic day, were stained immunohistochemically or immunofluorescently with antibodies against GATA4, Nkx2.5, insulin gene enhancer binding potein (ISL-1), α-smooth muscle actin and myosin heavy chain. The expression of GATA4 and phosphorylated histone H3(pHH3) were measured with Western blotting in the embryonic mouse hearts from ED11 to ED14. Results At ED7.5, positive cells for both transcription factors GATA4 and Nkx2.5 were found in the cardiogenic plate simultaneously. From ED8.5 to ED10, further expression of GATA4 and Nkx2.5 were detected in the splanchnic mesoderm of the dorsal wall of the pericardial cavity that constituted the second heart field(SHF). From ED11 to ED13, GATA4 and Nkx2.5 expressions were found descending from the reflection of dorsal pericardial wall with the distal pole of outflow tract to the base of the ascending aorta and pulmonary trunk. GATA4 expression was also detected in the foregut endoderm of ED11 to ED13 embryos. GATA4 and pHH3 expression in the embryonic mouse hearts reached the highest level between ED11 and ED13. Conclusion Transcription factor GATA4 shows the same expression pattern as that of Nkx2.5 during the early development of the embryonic mouse heart. Higher expression of GATA4 during crucial stages of heart morphogenesis suggests that GATA4 may play an important role in the proliferation of myocardial cells. The GATA4 expression in the foregut endoderm may be involved in the development of the SHF.

Key words

GATA4 / Heart-forming field / Endoderm / Immunohistochemistry / Western blotting / Mouse

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

CAI Yu-jin JING Ya* QIAO Ai-xiu YANG Yan-ping LI Hai-rong CUI Hui-lin ZHANG Tao. Association of transcriptional factor GATA4 expression patterns with arterial pole development of embryonic mouse heart[J]. Acta Anatomica Sinica. 2013, 44(2): 253-258 https://doi.org/10.3969/j.issn.0529-1356.2013.02.021

References

［1］ Sun Y, Liang X, Najafi N, et al. Islet1 is expressed in distinct cardiovascular lineages, including pacemaker and coronary vascular cells［J］. Dev Biol, 2007, 304(1):286-296.

［2］Takeuchi JK，Bruneau BG. Directed transdifferentiation of mouse mesoderm to heart tissue by defined factors［J］. Nature, 2009, 459(7247):708-711. 

［3］Pikkarainen S, Tokola H, Kerkel R, et al. GATA transcription factors in the developing and adult heart［J］. Cardiovasc Res, 2004, 63(2):196-207.

［4］Waldo KL,Kumiski DH,Wallis KT,et al.Conotruncal myocardium arises from a secondary heart field［J］. Development, 2001, 128(16):3179-3188.

［5］Kasahara H, Bartunkova S, Schinke M, et al. Cardiac and extracardiac expression of Csx/Nkx2.5 homeodomain protein［J］. Circ Res, 1998,

82(9):936-946.

［6］Cai CL, Liang X, Shi Y, et al. Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart［J］. Dev Cell, 2003, 5(6):877-889.

［7］ Molkentin JD, Lin Q, Duncan SA, et al. Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis［J］. Genes Dev, 1997, 11(8):1061-1072.

［8］Zeisberg EM, Ma Q, Juraszek AL, et al. Morphogenesis of the right ventricle requires myocardial expression of Gata4［J］. J Clin Invest, 2005, 115(6):1522-1531.

［9］ Dodou E, Verzi MP, Anderson JP, et al. Mef 2c is a direct transcriptional target of ISL1 and GATA factors in the anterior heart field during mouse embryonic development［J］. Development, 2004, 131(16):3931-3942.

［10］Rivera-Feliciano J, Lee KH, Kong SW, et al. Development of heart valves requires Gata4 expression in endothelialderived cells［J］. Development, 2006, 133(18):3607-3618.

［11］ Zhao R, Watt AJ, Battle MA, et al. Loss of both GATA4 and GATA6 blocks cardiac myocyte differentiation and results in acardia in mice［J］. Dev Biol, 2008, 317(2):614-619.

［12］ Holtzinger A, Rosenfeld GE, Evans T. Gata4 directs development of cardiac-inducing endoderm from ES cells［J］. Dev Biol, 2010, 337(1):63-73.

［13］Lough J, Sugi Y. Endoderm and heart development［J］. Dev Dyn, 2000, 217(4):327-342.

［14］ Wu ShSh, Jing Y, Yang YP, et al. Association of neural crest cells and ISL-1 positive cells with the development of the outflow tract of the mouse embryonic heart［J］.Acta Anatomica Sinica, 2011, 42(5): 684-689.(in Chinese)

武姗姗, 景雅, 杨艳萍, 等. 神经嵴细胞和胰岛因子1阳性细胞与小鼠胚胎心流出道发育的关系［J］. 解剖学报, 2011, 42(5): 684-689. 

［15］ Ma Q, Zhou B, Pu WT. Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity［J］. Dev Biol, 2008, 323(1):98-104.

［16］Xin M, Davis CA, Molkentin JD, et al. A threshold of GATA4 and GATA6 expression is required for cardiovascular development［J］. Proc Natl Acad Sci USA, 2006, 103(30):11189-11194.

［17］ Pu WT, Ishiwata T, Juraszek AL, et al. GATA4 is a dosage-sensitive regulator of cardiac morphogenesis［J］. Dev Biol, 2004, 275(1):235-244.

［18］ Holtzinger A, Evans T. GATA4 regulates the formation of multiple organs［J］. Development, 2005, 132(17):4005-4014. 