Ischemic postconditioning prevents cytochrome C release through up-regulation of p-Akt and protects neurons against ischemia insult&nbsp;

ZHANG Li-zhu  ZHOU Cai-feng  TU Jing-yi  ZHANG Xi  ZHU Ying  WANG Rui-min* 

doi:10.3969/j.issn.0529-1356.2013.02.002

Acta Anatomica Sinica ›› 2013, Vol. 44 ›› Issue (2) : 152-156. DOI: 10.3969/j.issn.0529-1356.2013.02.002

Ischemic postconditioning prevents cytochrome C release through up-regulation of p-Akt and protects neurons against ischemia insult 

ZHANG Li-zhu ^1,2 ZHOU Cai-feng ^1,3 TU Jing-yi¹ ZHANG Xi¹ ZHU Ying¹ WANG Rui-min^1*

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Abstract

Objective The goal of this study is to elucidate the neuro-protective effect and the possible mechanism of delayed ischemic postconditioning through observing the level of p-Akt and cytochrome C (Cyt C) in cytoplasm or mitochondria following global cerebral ischemia. Methods 40 Adult male Sprague-Dawley rats were subjected to global cerebral ischemia by four-vessel occlusion and were randomly divided into five groups: sham group, ischemia-reperfusion group (I/R), delayed ischemic postconditioning group (Post C), Vehicle group (Post C+Vehicle) and LY294002 group (Post C+LY294002). Cresyl violet staining was used to observe the surviving neurons of hippocampal CA1 region following global cerebral ischemia and western blot analysis was used to detect the level of p-Akt and Cyt C in both cytosolic and mitochondrial fraction. Results Delayed ischemic postconditioning protected the hippocampal CA1 region neurons against ischemia/referfusion injury and significantly increased the level of p-Akt in cytoplasm compared with I/R groups. Delayed ischemic postconditioning markedly prevented the release of Cyt C from mitochondria to cytoplasm and LY294002, an inhibitor of Akt up-stream kinase, abolished the positive role of delayed ischemic postconditioning. Conclusion Delayed ischemic postconditioning induces the Akt activation and prevents the release of Cyt C from mitochondria to cytoplasm, thereby blocks the hippocampal CA1 region neurons injury following global cerebral ischemia.

Key words

Global cerebral ischemia / Delayed ischemic postconditioning / Akt / Cytochrome C / Western blotting / Rat

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ZHANG Li-zhu ZHOU Cai-feng TU Jing-yi ZHANG Xi ZHU Ying WANG Rui-min* . Ischemic postconditioning prevents cytochrome C release through up-regulation of p-Akt and protects neurons against ischemia insult [J]. Acta Anatomica Sinica. 2013, 44(2): 152-156 https://doi.org/10.3969/j.issn.0529-1356.2013.02.002

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