Objective To determine the expression of mitogen activated protein kinase /extracellular signal regulated kinase 1(MAPK/ERK1) and signal transducer and activator of transcription 3(STAT3) in cystitis glandularis (CG) models of rats induced by exposure to lipopolysaccharide (LPS). Methods Twenty-four female Sprague-Dawley rats were used, 10 of which were catheterized and intravesically exposed to LPS for 8 weeks. Immunohistochemistry, RT-PCR and Western blotting methods were used to demonstrate the expression of ERK1 and STAT3 in sections of cystitis glandularis. Results Immunohistochemistry demonstrated that ERK1 and STAT3 were more intensive induced by LPS than those in the control. The relative expression of ERK1 and STAT3 of transcription were 1.99±0.12 and 1.71±0.08, respectively. 〖WTHZ〗Conclusion The increased ERK1 and STAT3 suggest that MAPK/ERK1-STAT signaling pathway may participate the pathological process of CG induced by LPS in r