Objective To establish an epithelial ovarian carcinoma SKOV3 cell line and nude mice models with transfecting mutant gene in FSHR 307 and 680 site in order to provide two experimental models for further study and treatment of epithelial ovarian carcinoma by follicle stimulating hormome(FSH). Methods FSHR RNA from the primary culture of granular cells in human ovary were extracted. Recombination protein about FSHR and mutant point in FSHR 307 and 680 site were constructed and transfected to SKOV3 cell line. SKOV3, SKOV3 cells with FSHR and mutant point in FSHR 307 and 680 site cells were inoculated subcutaneously in female nude mice to form solid tumors. Pathological examinations were performed after 8 weeks. Results An epithelial ovarian carcinoma SKOV3 cell line and nude mice models with transfecting mutant gene in FSHR 307 and 680 site were established. Conclusion Establishing an epithelial ovarian carcinoma with transfecting mutant gene in FSHR 307 and 680 site in vitro and in vivo offers two considerable models to explore and treat epithelial ovarian carcinoma by FSH.