Objective To investigate effects of formaldehyde on endogenous metabolites of urine. Methods The urinary samples of the formaldehyde contact group and control group were analyzed by UPLC –Q-TOF/MS, combined with principal component analysis(PCA). The overall disease pattern of the metabolic network was established based on metabolic profiles in different groups. Results The different trajectory of urine was visually noted from metabolic profiles between 2 groups. Metabolomics appeared to be suitable for toxicity studies of formaldehyde, and a biomarker structure was identified. Conclusion The science connotation of the toxicity of formaldehyde may be elucidated by Metabonomics, according to metabolites variance in certain disease