Objective To investigate whether ANXA5 could be used as a diagnose marker for human uterine cervical squamous cell carcinoma. Methods The blood of 38 normal women and 48 uterine cervical squamous cell carcinoma patients was collected. The serum and red blood cells were obtained by low speed centrifugation. The content of ANXA5 and SCCAg was detected by ELISA method and RT-PCR was used to determine the mRNA expression of ANXA5 The data of the two groups were analyzed by t test and the relativity analysis was done between ANXA5 and SCCAg. Results According to ELISA method, the contents of both ANXA5 and SCCAg in the serum of uterine cervical carcinoma patients were higher than that of normal women（EM>P /EM><0.05). RT-PCR results showed that ANXA5 mRNA level in the patients was higher than that of normal（EM>P/EM> <0.05）. The relativity analysis showed that ANXA5 and SCCAg had a positive correlation, but the positive ratio of ANXA5 was higher than that of SCCAg. BR>Conclusion The content of ANXA5 in the uterine cervical carcinoma patients increased significantly with high speciality and sensitivity and might be used as a