Objective To explore the method of designing and manufacturing the mandibular condyle scaffold individually by rapid prototyping technologies and reverse engineering.Methods Cranial CT image data were processed in Mimics software for reconstruction of one side of ramus of mandible, and were inputted into Solidworks software to edit by the format of stl. A negative mold of the mandibular condyle scaffold was obtained. The mold was fabricated by resin materials utilizing rapid prototyping. Biomaterials were filled into the resin mold. When the materials were cured, we eliminated the resin mold and acquired a multi-pores three dimensional mandibular condyle scaffold model. The general morphology and microstructure of the scaffold model were observed by scanning electron microscopy (SEM). Results The mandibular condyle scaffold made of biomaterials was accordance with the one our computer designed. SEM observation revealed that the model was made of collagen in the cartilage-like layer and calcium phosphate cement/poly (lactic- co-glycolic acid) in the bone-like layer. Conclusion It is feasible to fabricate mandibular condyle scaffold individually by using reverse engineering and rapid prototyping technology.