Objective To study the development of splenium corporis callosi in children. Methods One hundred and twenty healthy children (5 days-14 years) were classified into five groups: group 1 (≤1 year old), group 2 (＞1-3 years old), group 3 (＞3-6 years old), group 4 (＞6-10 years old) and group 5 (＞10-14 years old), and underwent conventional magnetic resonance imaging (MRI) and diffusion tensor imaging (DTI). Fibers were reconstructed through raw data by diffusion tensor tractography (DTT) and were analyzed statistically. Results The density of fibers of splenium corporis callosi in young children, school age children and adolescence period children were 432 (256-512), 580 (355-710) and 754 (580-900) respe