Objective To determine the anatomical basis of an algorithm to safely elevate the deep inferior epigastric artery perforator (DIEP) flap. Methods Eight fresh bodies underwent whole body gelatine/lead oxide injection, CT scanning，3D-reconstrution and the vascular cross-linking across the midline were observed. Specimens were dissected by layers. Angiography and photography were used to document the precise course, size, location, and type of individual perforators in the anteriolateral abdominal region. The surface areas of cutaneous territories and perforator zones were measured and calculated with Photoshop and Scion Image. Results The inferior epigastric artery was present in 100% of specimens. An average of (4.8±1.7) perforators with a diameter of (0.7±0.2) mm perforators were located, with a perforator zone of 33 cm2, the flap size was 20cm×15cm.Conclusion The DIEP reliably perfuses the anteriolateral abdominal region; the vascular cross-linking across the midline of the lower abdominal flap is rich. It offers a large quantity of transverse DIEP flap on a pedicle of large diameter. The la