Objective To investigate the temporal and spatial expression of Wnt2b/β-catenin/C-myc signaling pathway in mouse embryonic heart. Methods The mouse embryonic hearts taken from gravidity 10 days(G10d), G12.5d, G15d, G17d, G18.5d, and postnatal day(P0d) were used. Immunohistochemistry, RT\|PCR and Western blotting were performed to detect the expressions of Wnt2b, β-catenin and C-myc during different periods of mouse embryonic heart development. Results With the progress of the embryonic heart development, the expressions of Wnt2b, β-catenin and C-myc at the mRNA and protein levels were decreasing gradually, and the mRNA expression of Wnt2b,β-catenin and C-myc showed that they were interrelated statistically (EM>P/EM><0.05).Conclusion These results suggest that Wnt2b/β-catenin/C-myc signaling pathway play important roles in regulating the development of the embryonic heart. And it can predict that C-my