Abstract
Objective To construct recombinant phospholipid transfer protein(PLTP) cell model and evaluate the effect mechanisms of Barbata flavonoids(BF),through the test of their suppressive function of PLTP in Bel-7402 cells. MethodsPLTP cDNA flag was amplified with reverse transcription-polymerase chain reaction(RT-PCR), PLTP/p3XFLAG-MV-14 was constructed and transfected into Bel-7402 cells. The Bel-7402 cells influenced by Barbata flavonoids were analyzed by immunohistochemical assay. ResultThe expression of PLTP was decreased in Bel-7402 cells treated with 160 mg/L Barbata flavonoids after 24hours,48hours,72hours as compared with the controls（EM>Ｐ/EM><0.05, EM>Ｐ/EM><0.05,EM>Ｐ/EM><0.05）.Under high, middle, low-dose BF, treated after 24hours,48hours,72hours, the expression of PLTP had no significant difference as compared with model group（EM>P/EM>>0.05）. ConclusionWe used gene recombination technology to build recombinant gene cell Bel-7402
Key words
Barbata flavonoids /
Bel-7402 /
Phospholipid transfer protein /
Recombinant Gene /
RT-PCR /
Western blotting /
Immunohistochemisitry
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Effect of barbata flavonoids on the expression of PLTP in the recombinant PLTP gene cells[J]. Acta Anatomica Sinica. 2011, 42(2): 185-189 https://doi.org/10.3969/j.issn.0529-1356.2011.02.009
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