Objective To detect the expression of annexin A7 in human uterine cervical squamous cell carcinoma tissues and normal tissues. Methods SP immunohistochemistry was used to observe the differential expression of annexin A7 between carcinoma and normal paraffin imbedded specimen, each 25 cases. Western blotting and RT-PCR were used to observe the differential expression of annexin A7 between human uterine cervical squamous cell carcinoma and normal fresh tissues,each 25 cases. Results The annexin A7 expression in the tumor tissues was much higher than that of the normal tissues at the protein and the mRNA levels (P<0.05). Conclusion The expression of annexin A7 is upregulated in human uterine cervical squamous cell carcinoma tissues and it might be a new valuable tumor marker.