Objective The eight enzymes’ levels in serum from Panthera tigris amureusis （ P. t. amureusis ） were determined. The data wouldl be accumulated for the growth, reproduction and disease diagnosis and treatment of young P. t. amureusis , as well as types of tiger subspecies genetic and evolutional relationship studies. Methods Eight enzymes’ biological indexes of 13 individuals had been measured and analyzed in P. t. amureusis by HITCH 7200-20 automated chemistry analyzer, respectively between male and female young P. t. amureusis , and between young P. t. amureusis and adult. Results Aspartate aminotransferase(AST) was (29.31±5.88)IU/L, lactate dehydrogenase enzyme(LDH) was (267.08±76.40)IU/L,α-hydroxybutyrate dehydrogenase(α-HBDH) was (149.38±54.07)IU/L, gamma-glutamyl transpeptidase(GGT) was (2.92±1.94)IU/L, creatine kinase(CK) was (284.77±132.02)IU/L, creatine kinase isoenzyme(CKMB) was (390.62±145.70)IU/L and actate dehydrogenase isoenzyme(LDI) was (11.11±7.08)IU/L.Conclusion There are no differences in