P>Objective To study the expression of microtubule-associated protein-2(MAP-2) and neurofilament-200(NF-200) in cortex, hippocampus and amygdaloid body of rat after sleep deprivation(SD). Methods SD model was made by modified multiple platform method（MMPM）, rats were divided into sleep deprivation group(SD),tank control group(TC), cage control group(CC). The sleep deprivation group included seven time-length groups (sleep deprivation 6 hours,12 hours,1 day,2 days,3 days,5 days and 7 days). Five rats were assigned to each group. The expression of MAP-2 and NF-200 were detected by immunohistochemical method. Results At the 5th day and 7th day, we found that the density of MAP-2 and NF-200 positive neuron decreased in cortex, CA1,CA2,CA3,dentate gyrus and amygdaloid body（P0.05，P0.01). Conclusio