Objective To explore the correlation of unknown geneEM> AI408225/EM> and humoral immunity involved in rat regenerating liver. Methods Isolation of rat 8 liver cell types, detection of their genes expression change were accorded to Percoll density gradient centrifugation combined with immune magnetic beads separation method, and their genes sequence homoeology and coexpression relation were analyzed by Microsoft Excel, BLAST software, and the prediction physiological activities were analyzed by bioinformatics and systematic biology. Results A total of 93 humoral immunityass ociated genes yielded the meaningful expression changes in liver regeneration, the corresponding gene numbers in 8 liver cell types included hepatocytes, bile duct epithelial cells, oval cells, hepatic stellate cells, sinus endothelial cells, Kupffer cells, pit cells and dendritic cells were 33, 46, 17, 59, 48, 35, 53, 68 Among thatEM>AI408225/EM>and EM>cd/EM>4 are homology and coexpression. Conclusion The rat liver regeneration is closely associated with humoral immunity, and AI408225 participat